Quantitation of global histone post-translational modifications reveal anti-inflammatory epigenetic mechanisms of liquiritigenin based on the optimized super-SILAC strategy
文献类型：期刊论文
| 作者 | Liu, Ping2,3; Zhang, Jun3,4; Zhang, Jingdan5,6; Yuan, Yucheng3,4; Liu, Zhiqing3; Chen, Sixian2,3; Chen, Kaifeng3,4; Dong, Li6; Cheng, Zhiyuan4,7; Zhang, Yinan2 |
| 刊名 | FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
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| 出版日期 | 2025-03-27 |
| 卷号 | 13页码:15 |
| 关键词 | histone post-translational modifications super-SILAC liquiritigenin inflammation epigenetics PPAR |
| ISSN号 | 2296-634X |
| DOI | 10.3389/fcell.2025.1566567 |
| 通讯作者 | Tan, Minjia(mjtan@simm.ac.cn) ; Zhao, Wensi(zhao_wensi@tongji.edu.cn) ; Xie, Dong(xiedong@tongji.edu.cn) |
| 英文摘要 | Liquiritigenin (LIQ) is a dihydroflavonone monomer compound with a planar ring structure that exhibits potent anti-inflammatory activity. The post-translational modifications (PTMs) of histones are closely associated with inflammatory diseases. To explore the relationships between the anti-inflammatory effects and epigenetic regulatory mechanisms of LIQ, we optimized the super stable isotope labeling by amino acids in cell culture (super-SILAC) method combined with a compound stimulation strategy. Moreover, we evaluated the identification coverage and demonstrated high reliability as well as reproducibility of the optimized method at both the peptide and cellular lysate levels, which are promising for elucidating disease pathology and drug mechanisms. We further applied the method to a system-wide characterization of histone PTMs in M1 macrophages treated with LIQ. The quantitative results showed that H4K5ac, H4K16ac, H3K9ac, H3K27ac, and H2BK12ac are significantly upregulated. Transcriptome analysis revealed that LIQ could exert anti-inflammatory effects by modulating the histone PTMs and regulating gene expressions through the peroxisome proliferator-activated receptor (PPAR) signaling pathway. Collectively, we provide a sensitive and universal strategy for research on the epigenetic mechanisms of natural products as well as facilitate epigenetic understanding of LIQ in inflammatory therapies. |
| WOS关键词 | MACROPHAGE POLARIZATION ; NICOTINAMIDE ; METABOLISM ; FLAVONOIDS ; INFLAMMATION ; ACETYLATION ; INHIBITION ; CELLS ; YEAST ; MICE |
| 资助项目 | National Key Research and Development Program of China[2024YFC2815903] ; Natural Science Foundation of China[22225702] ; Natural Science Foundation of China[82272943] ; Program of Shanghai Academic Research Leader[22XD1420900] ; Science and Technology Commission of Shanghai Municipality[21Y11913400] |
| WOS研究方向 | Cell Biology ; Developmental Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001462613900001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316983]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Tan, Minjia; Zhao, Wensi; Xie, Dong |
| 作者单位 | 1.Tongji Univ, Shanghai Pulm Hosp, Sch Med, Dept Thorac Surg, Shanghai, Peoples R China 2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing, Jiangsu, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou, Peoples R China 6.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan, Peoples R China 7.Chinese Acad Sci, Acad Math & Syst Sci, State Key Lab Math Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ping,Zhang, Jun,Zhang, Jingdan,et al. Quantitation of global histone post-translational modifications reveal anti-inflammatory epigenetic mechanisms of liquiritigenin based on the optimized super-SILAC strategy[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2025,13:15. |
| APA | Liu, Ping.,Zhang, Jun.,Zhang, Jingdan.,Yuan, Yucheng.,Liu, Zhiqing.,...&Xie, Dong.(2025).Quantitation of global histone post-translational modifications reveal anti-inflammatory epigenetic mechanisms of liquiritigenin based on the optimized super-SILAC strategy.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,13,15. |
| MLA | Liu, Ping,et al."Quantitation of global histone post-translational modifications reveal anti-inflammatory epigenetic mechanisms of liquiritigenin based on the optimized super-SILAC strategy".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 13(2025):15. |
入库方式： OAI收割
来源：上海药物研究所
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