Screening and functional characterization of nanobodies targeting the transferrin receptor
文献类型：期刊论文
| 作者 | Ding, Yuting6; Jia, Li6; Geng, Qifubo3; Liu, Yan1,2; Guo, Shaojue6; Zhao, Shuaiying6; Kong, Yingying1,2; Jin, Quanfang4; Xu, Guangxu5; Xu, Jianfeng6 |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2025-07-01 |
| 卷号 | 231页码:7 |
| 关键词 | Transferrin receptor (TfR1) Nanobody screening Cancer therapy |
| ISSN号 | 1046-5928 |
| DOI | 10.1016/j.pep.2025.106702 |
| 通讯作者 | Jin, Quanfang(2439396821@qq.com) ; Xu, Guangxu(xgx813@163.com) ; Xu, Jianfeng(jfxu@shou.edu.cn) |
| 英文摘要 | The transferrin receptor (TfR1) mediates the cellular uptake of iron and other molecules, playing a vital role in hematology and tumor growth. Nanobodies (NBs) targeting TfR1 offer promising therapeutic potential due to their small size, high specificity and stability. However, rapid identification of effective nanobodies remains challenging.In this study, the truncated extracellular fragment of human TfR1 was expressed in a prokaryotic system and purified. Immunized camelids provided a source for nanobody libraries, which were screened using phage display and high-throughput strategies to identify candidates with specific TfR1 binding.NB 2D7 with nanomolar-level dissociation constants (KD) were successfully identified.The analysis of Cell Counting Kit-8 (CCK8) experiments indicates that the combined treatment of NB2D7 with FeCl3 can reduce the survival rate of LoVo cells.This research establishes an efficient platform for anti-TfR1 nanobody screening and highlights the therapeutic potential of these nanobodies in cancer treatment and iron metabolism disorders. |
| WOS关键词 | BLOOD-BRAIN-BARRIER ; ANTIBODY ; CANCER ; TFR1 ; EXPRESSION |
| 资助项目 | Shanghai Kailuo Biotechnology Co., Ltd. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001460989800001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316989]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Jin, Quanfang; Xu, Guangxu; Xu, Jianfeng |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Receptor Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Henan Univ, Coll Pharm, Kaifeng 475000, Henan, Peoples R China 3.Minzu Univ China, Sch Informat Engn, Beijing 100081, Peoples R China 4.Shanghai Baoshan Hosp Integrated Tradit Chinese &, Shanghai 201900, Peoples R China 5.Shanghai Univ Med & Hlth Sci, Affiliated Peoples Hosp 6, Dept Gynecol, South Campus, Shanghai 200233, Peoples R China 6.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Biopharmaceut, Shanghai 201306, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, Yuting,Jia, Li,Geng, Qifubo,et al. Screening and functional characterization of nanobodies targeting the transferrin receptor[J]. PROTEIN EXPRESSION AND PURIFICATION,2025,231:7. |
| APA | Ding, Yuting.,Jia, Li.,Geng, Qifubo.,Liu, Yan.,Guo, Shaojue.,...&Xu, Jianfeng.(2025).Screening and functional characterization of nanobodies targeting the transferrin receptor.PROTEIN EXPRESSION AND PURIFICATION,231,7. |
| MLA | Ding, Yuting,et al."Screening and functional characterization of nanobodies targeting the transferrin receptor".PROTEIN EXPRESSION AND PURIFICATION 231(2025):7. |
入库方式： OAI收割
来源：上海药物研究所
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