Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats
文献类型：期刊论文
| 作者 | Bai, Yang1; Wang, Rui1; Jin, Can2,3; Wang, Lili1; Tang, Yun1; Wang, Han1; Ding, Kan2,3 ; Cai, Shanjun1
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| 刊名 | FRONTIERS IN PHARMACOLOGY
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| 出版日期 | 2025-04-09 |
| 卷号 | 16页码:13 |
| 关键词 | |
| DOI | 10.3389/fphar.2025.1499420 |
| 通讯作者 | Ding, Kan(dingkan@simm.ac.cn) ; Cai, Shanjun(caishanjun@163.com) |
| 英文摘要 | Retinal neovascularization, a pathological form of angiogenesis, is a leading cause of blindness. This study investigated the effects of Dendrobium sulfate polysaccharide JCS1S2, derived from Dendrobium chrysogenum, on oxygen-induced retinopathy (OIR) in rat models and M & uuml;ller cells of the rat retina. To this end, we established an OIR rat model and divided the rats into three primary groups, namely, Group I (control), Group II (OIR), and Group III (OIR + JCS1S2). Group III was further subdivided into three subgroups treated with different concentrations of JCS1S2 (10 mu g/mu L, 20 mu g/mu L, and 40 mu g/mu L). After finding the optimal concentration of JCS1S2 by ADP and HE, PCR, Western blot and transcriptome sequencing were used to analyze the role of JCS1S2 in M & uuml;ller cells of OIR rats and rat retinas. ADP and hematoxylin and eosin (HE) staining revealed that JCS1S2 dose-dependently inhibited retinal neovascularization. Quantitative polymerase chain reaction (qPCR) and Western blot analyses showed significant downregulation of vascular endothelial growth factor A (VEGF-A), VEGF-B, VEGF-D, VEGF receptor 1 (VEGF-R1), and VEGF receptor 2 (VEGF-R2) following JCS1S2 treatment. Transcriptome analysis suggested that JCS1S2 may suppress the activation of the Toll-like receptor (TLR) signaling pathway, regulate the expression of genes associated with endothelial activation and angiogenesis, and participate in the inflammatory and metabolic pathways of the retina. Western blotting data indicate that JCS1S2 can markedly reduce abnormal retinal angiogenesis and M & uuml;ller cell activation in OIR rats through the TLR4/p-NF-kappa B/VEGF pathway, JCS1S2 may have the potential as a therapeutic agent for retinal neovascularization. |
| WOS关键词 | ENDOTHELIAL GROWTH-FACTOR ; FACTOR VEGF ; MACULAR DEGENERATION ; RANIBIZUMAB ; BEVACIZUMAB ; PROTECTS ; NEURONS ; BINDING |
| 资助项目 | National Natural Science Foundation of China[31871261] ; Guizhou Science and Technology Cooperation[2023] ; Zunyi Science and Technology |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001472352000001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/317503]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ding, Kan; Cai, Shanjun |
| 作者单位 | 1.Zunyi Med Univ, Affiliated Hosp,Special Key Lab Ocular Dis Guizhou, Guizhou Eye Hosp,Dept Ophthalmol, Guizhou Prov Branch,Natl Eye Dis Clin Res Ctr, Zunyi, Guizhou, Peoples R China 2.Univ Chinese Acad Sci, Shanghai Inst Mat Med, Chinese Acad Sci, Key Lab Receptor Res,Glycochem & Glycobiol Lab, Beijing, Peoples R China 3.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Yang,Wang, Rui,Jin, Can,et al. Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats[J]. FRONTIERS IN PHARMACOLOGY,2025,16:13. |
| APA | Bai, Yang.,Wang, Rui.,Jin, Can.,Wang, Lili.,Tang, Yun.,...&Cai, Shanjun.(2025).Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats.FRONTIERS IN PHARMACOLOGY,16,13. |
| MLA | Bai, Yang,et al."Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats".FRONTIERS IN PHARMACOLOGY 16(2025):13. |
入库方式： OAI收割
来源：上海药物研究所
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