ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13
文献类型：期刊论文
| 作者 | Liu, Hongqin1; Chen, Xi7,8; Wang, Hanlin5,6; Zhuang, Guanglei3,4; Zhu, Zheng-Jiang2,7; Zhuang, Min1 |
| 刊名 | JOURNAL OF CELL BIOLOGY
 |
| 出版日期 | 2025-04-17 |
| 卷号 | 224期号:6页码:21 |
| ISSN号 | 0021-9525 |
| DOI | 10.1083/jcb.202407198 |
| 通讯作者 | Zhu, Zheng-Jiang(jiangzhu@sioc.ac.cn) ; Zhuang, Min(zhuangmin@shanghaitech.edu.cn) |
| 英文摘要 | Peroxisomes are integral metabolic organelles involved in both catabolic and anabolic processes in humans, with defects linked to diseases. The functions of peroxisomes are regulated at transcriptional, translational, and posttranslational levels. In this study, we employed the CRISPR/Cas9-based screening of a ubiquitin ligase library to identify regulators of human peroxisomes. We discovered that ZBTB17 (MIZ1) plays a role in regulating the import of proteins into peroxisomes. Independent of its ubiquitin ligase activity, ZBTB17/MIZ1 operates as a transcription factor to modulate the expression of key importer PEX13, influencing the localization of peroxisomal enzymes. Furthermore, metabolomic profiling reveals that knockdown of ZBTB17 or PEX13 results in similar metabolic alterations, with downregulated purine synthesis. Collectively, we identify ZBTB17 as a key regulator of peroxisomal protein import, thereby affecting peroxisomal function and nucleotide metabolism. Our findings provide insights into the multifaceted regulation of peroxisomes in complex human cells and shed light on the molecular mechanisms underlying ZBTB17's role as a transcriptional regulator. |
| WOS关键词 | R/BIOCONDUCTOR PACKAGE ; PROTEIN IMPORT ; ZINC-FINGER ; MEMBRANE ; RECEPTOR ; PATHWAY ; DISORDERS ; PEX5P ; ANNOTATION ; DOMAIN |
| 资助项目 | National Key R&D Program of China ; School of Life Science and Technology at ShanghaiTech University |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001469216400001 |
| 出版者 | ROCKEFELLER UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/317512]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhu, Zheng-Jiang; Zhuang, Min |
| 作者单位 | 1.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 2.Shanghai Key Lab Aging Studies, Shanghai, Peoples R China 3.Shanghai Jiao Tong Univ, Ren Ji Hosp, Sch Med, Shanghai Key Lab Gynecol Oncol, Shanghai, Peoples R China 4.Shanghai Jiao Tong Univ, Ren Ji Hosp, Shanghai Canc Inst, Sch Med,Dept Obstet & Gynecol,State Key Lab Syst M, Shanghai, Peoples R China 5.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 7.Chinese Acad Sci, Interdisciplinary Res Ctr Biol & Chem, Shanghai Inst Organ Chem, Shanghai, Peoples R China 8.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hongqin,Chen, Xi,Wang, Hanlin,et al. ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13[J]. JOURNAL OF CELL BIOLOGY,2025,224(6):21. |
| APA | Liu, Hongqin,Chen, Xi,Wang, Hanlin,Zhuang, Guanglei,Zhu, Zheng-Jiang,&Zhuang, Min.(2025).ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13.JOURNAL OF CELL BIOLOGY,224(6),21. |
| MLA | Liu, Hongqin,et al."ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13".JOURNAL OF CELL BIOLOGY 224.6(2025):21. |
入库方式： OAI收割
来源：上海药物研究所
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