Bromine-Substituted Tricyanofuran-Based Fluorogenic Probes for the Sensitive Detection of Glycosidases under Acidic pH
文献类型：期刊论文
| 作者 | Zhao, Xue-Ru1,2,5; Si, Fang-Yu1,2; Wang, Jing-Bo1,2; Wang, Ning1,2; Hu, Xi-Le1,2; Zang, Yi3; Wang, Chengyun1,2; Li, Jia5 ; He, Xiao-Peng1,2,4
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| 刊名 | CHEMBIOCHEM
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| 出版日期 | 2025-04-24 |
| 页码 | 6 |
| 关键词 | cell imaging fluorogenic glycosidase pH probes |
| ISSN号 | 1439-4227 |
| DOI | 10.1002/cbic.202400736 |
| 通讯作者 | Wang, Chengyun(cywang@ecust.edu.cn) ; Li, Jia(jli@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn) |
| 英文摘要 | Glycosidases participate in modulating a number of biological processes, and their overexpression is a hallmark of several human diseases. Considering the localization of glycosidases predominantly in the lysosomes, it is imperative to develop molecular probes that are resistant to interference from acidic pH. Herein, tricyanofuran-based long-wavelength fluorogenic probes are synthesized, and it is demonstrated that the introduction of bromine to the ortho-position of the phenol alcohol of the probe substantially enhances its fluorescence over an acidic pH range of 3.0-10.0. These probes, when modified with glycosyl substrates, have proved applicable for the sensitive fluorogenic detection of glycosidases under acidic pH, and fluorescence-based imaging of endogenous beta-galactosidase and beta-glucosidase activities in live cells. The imaging results are validated by quantitative real-time polymerase chain reaction and immunoblotting. |
| WOS关键词 | FLUORESCENCE PROBE ; CELLULAR SENESCENCE ; FUCOSIDASE ; CARCINOMA ; BIOMARKER ; CELLS |
| 资助项目 | National Natural Science Foundation of China (NSFC) ; Science and Technology Commission of Shanghai Municipality[24DX1400200] ; International Cooperation Program of Shanghai Science and Technology[23490711600] ; Fundamental Research Funds for the Central Universities[222201717003] ; Programme of Introducing Talents of Discipline to Universities[B16017] ; National Natural Science Foundation of Shanghai Science and Technology[24ZR1415400] ; Shanghai Oriental Talents youth Program[QNKJ2024010] ; Shanghai Xuhui District Hospital Local Cooperation Project[23XHYD-20] ; Open Funding Project of the State Key Laboratory of Fine Chemicals, Dalian University of Technology[KF 2402] ; State Key Laboratory of Drug Research[SKLDR-2023-KF-10] ; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University ; Ministry of Education Key Laboratory on signaling Regulation and Targeting Therapy of Liver Cancer (Naval Medical University)[2023-MEKLLC-MS/ZD-00] ; Shandong Laboratory Program[SYS202205] ; Research Center of Analysis and Test of East China University of Science and Technology ; [92253306] ; [82130099] ; [22477030] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001473319200001 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/317518]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wang, Chengyun; Li, Jia; He, Xiao-Peng |
| 作者单位 | 1.East China Univ Sci, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Res Lab Precis Chem & Mol EngineeringFer, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China 2.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China 3.Lingang Lab, Shanghai 201203, Peoples R China 4.Inst Eastern Hepatobiliary Surg Hosp, Natl Ctr Liver Canc, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Xue-Ru,Si, Fang-Yu,Wang, Jing-Bo,et al. Bromine-Substituted Tricyanofuran-Based Fluorogenic Probes for the Sensitive Detection of Glycosidases under Acidic pH[J]. CHEMBIOCHEM,2025:6. |
| APA | Zhao, Xue-Ru.,Si, Fang-Yu.,Wang, Jing-Bo.,Wang, Ning.,Hu, Xi-Le.,...&He, Xiao-Peng.(2025).Bromine-Substituted Tricyanofuran-Based Fluorogenic Probes for the Sensitive Detection of Glycosidases under Acidic pH.CHEMBIOCHEM,6. |
| MLA | Zhao, Xue-Ru,et al."Bromine-Substituted Tricyanofuran-Based Fluorogenic Probes for the Sensitive Detection of Glycosidases under Acidic pH".CHEMBIOCHEM (2025):6. |
入库方式： OAI收割
来源：上海药物研究所
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