Screening of botanical drugs reveals the potential anti-human adenovirus activity of berberine
文献类型：期刊论文
| 作者 | Cao, Junyuan4,5,6; Zhang, Hao2,6; Zhang, Jixiang4,5; Wang, Jinlin1; Li, Chen2,6; Ma, Jin2,6; Ye, Zhengyu4,5; Zheng, Yunting2,6; Liu, Hong1,3 ; Xiao, Gengfu4,5
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| 刊名 | ANTIVIRAL RESEARCH
 |
| 出版日期 | 2025-05-01 |
| 卷号 | 237页码:9 |
| 关键词 | Human adenovirus Antiviral drug Berberine High-throughput screening |
| ISSN号 | 0166-3542 |
| DOI | 10.1016/j.antiviral.2025.106105 |
| 英文摘要 | Human adenovirus (HAdV) is a significant viral pathogen that causes severe acute respiratory infections (SARIs) in children and immunocompromised patients. Currently, no specific treatment options are available for HAdV infections. This study used a green fluorescence protein-based, high-throughput screening (HTS) assay on a botanical drug library containing 3697 botanical compounds to identify agents that could inhibit HAdV. Four compounds with anti-HAdV-C5 activity in the low-micromolar range were identified and inhibited other wildtype HAdVs known to cause SARIs. Among these compounds, 13-methylberberine chloride presented the highest select index values. Berberine is a commercially available natural product-derived isoquinoline alkaloid with multiple pharmacological effects and is widely used in Asian countries. We systematically evaluated the anti-HAdV activity of six berberine-derived compounds in vitro and performed a time-of-drug-addition assay to explore their antiviral modes of action. Mechanistic studies revealed that berberine and its analogs inhibit HAdV replication by downregulating the MAPK signaling pathway, particularly ERK activation, which is crucial for viral replication and progeny production. Our findings suggest that berberine is a promising candidate for the development of anti-HAdV therapies. |
| WOS关键词 | INHIBITION ; DNA ; BRINCIDOFOVIR ; REPLICATION ; CIDOFOVIR ; KINASE |
| 资助项目 | National Natural Science Foundation of China[82130105] ; Science Fund for Distinguished Young Scholars of Anhui Province[2022CFA099] ; Guangdong Province Introduction of Innovative RD Team[GZNL2024A01008] ; [22107107] |
| WOS研究方向 | Pharmacology & Pharmacy ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:001435882600001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316493]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Xiao, Gengfu; Dai, Wenhao; Zhang, Leike |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.HuaWu Biotech Wuhan, Wuhan 430200, Peoples R China 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Virol, Ctr Biosafety Mega Sci, State Key Lab Virol, Wuhan 430071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Hubei Jiangxia Lab, Wuhan 430200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Junyuan,Zhang, Hao,Zhang, Jixiang,et al. Screening of botanical drugs reveals the potential anti-human adenovirus activity of berberine[J]. ANTIVIRAL RESEARCH,2025,237:9. |
| APA | Cao, Junyuan.,Zhang, Hao.,Zhang, Jixiang.,Wang, Jinlin.,Li, Chen.,...&Zhang, Leike.(2025).Screening of botanical drugs reveals the potential anti-human adenovirus activity of berberine.ANTIVIRAL RESEARCH,237,9. |
| MLA | Cao, Junyuan,et al."Screening of botanical drugs reveals the potential anti-human adenovirus activity of berberine".ANTIVIRAL RESEARCH 237(2025):9. |
入库方式： OAI收割
来源：上海药物研究所
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