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Direct Preparation of Site-Specific Antibody-Drug Conjugates with Unpurified Antibodies in Culture Medium

文献类型：期刊论文

作者Lu, Mengru1,2; Zeng, Yue1,2; Hang, Jiaying1,2; Shi, Wei1,2; Huang, Wei1,2; Tang, Feng1,2
刊名CHEMBIOCHEM
出版日期2025-04-04
页码7
关键词cellular expression systems K248 site-specific ADCs one-pot
ISSN号1439-4227
DOI10.1002/cbic.202401082
英文摘要In comparison with traditional chemotherapeutic drugs, antibody-drug conjugates (ADCs) have been extensively employed in cancer therapy due to their high selectivity and low systemic toxicity. However, the structural complexity of ADCs makes its preparation process more intricate and time-consuming. Conventional ADC production involves multistep purification processes, which significantly increase the costs. Herein, an approach for the direct preparation of site-specific ADCs in an antibody expression system that is derived from the reported K248 site-specific modification technology is demonstrated. This strategy requires only a single purification step to obtain site-specific ADCs, thereby greatly shortening the preparation cycle of ADCs. The preparation of site-specific ADCs in the expression system of Sacituzumab is successfully achieved. The resulting ADCs exhibited comparable physicochemical properties and activities to those obtained through the conventional two-step purification strategy. The approach demonstrates high compatibility with various linkers and antibodies, offering a streamlined methodology for the preparation of site-specific ADCs.
资助项目National Natural Science Foundation of China[82325045] ; National Natural Science Foundation of China[22422705] ; National Natural Science Foundation of China[92478204] ; National Natural Science Foundation of China[22337003] ; National Natural Science Foundation of China[22277126] ; National Natural Science Foundation of China[82204183] ; Natural Science Foundation of China (NSFC)[22YF1457400] ; Natural Science Foundation of China (NSFC)[24YF2755200] ; Shanghai Sail Program[GZC20241827] ; Postdoctoral Fellowship Program (Grade C) of China Postdoctoral Science Foundation ; SANOFI Scholarship Program
WOS研究方向Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:001458722700001
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/316926]  
专题新药研究国家重点实验室
通讯作者Shi, Wei; Huang, Wei; Tang, Feng
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Lu, Mengru,Zeng, Yue,Hang, Jiaying,et al. Direct Preparation of Site-Specific Antibody-Drug Conjugates with Unpurified Antibodies in Culture Medium[J]. CHEMBIOCHEM,2025:7.
APA Lu, Mengru,Zeng, Yue,Hang, Jiaying,Shi, Wei,Huang, Wei,&Tang, Feng.(2025).Direct Preparation of Site-Specific Antibody-Drug Conjugates with Unpurified Antibodies in Culture Medium.CHEMBIOCHEM,7.
MLA Lu, Mengru,et al."Direct Preparation of Site-Specific Antibody-Drug Conjugates with Unpurified Antibodies in Culture Medium".CHEMBIOCHEM (2025):7.

入库方式： OAI收割

来源：上海药物研究所

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