Chemical structure and anti-inflammatory effects on intestinal epithelial cells of a novel mannogalactan purified from Typhonium giganteum Engl.
文献类型：期刊论文
| 作者 | Luo, Hongchen2,5,6,7; Lai, Zhengyang3,4,5; Shi, Lexue1,4,5; Hu, Yulong2,6,7; Jin, Can4,5; Ding, Kan1,2,3,4,5,6,7
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| 刊名 | CARBOHYDRATE RESEARCH
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| 出版日期 | 2025-06-01 |
| 卷号 | 552页码:13 |
| 关键词 | Typhonium giganteum Engl. Polysaccharide NCM460cells Anti-inflammation NF-kappa B MAPK |
| ISSN号 | 0008-6215 |
| DOI | 10.1016/j.carres.2025.109467 |
| 英文摘要 | Inflammatory bowel disease (IBD) remains a pressing global health challenge due to its complex pathogenesis and limited treatment efficacy. Mannogalactans, abundant in certain algae and edible fungi, are recognized for their diverse biological functions, particularly their potent anti-inflammatory effects. Motivated by these findings, we hypothesized that Typhonium giganteum Engl. may contain structurally analogous mannogalactans with therapeutic potential against enteritis. To investigate, we isolated and purified a homogeneous polysaccharide, BFZ213, using hot water extraction, ethanol precipitation, and chromatographic techniques. Comprehensive structural analysis, employing methylation analysis, partial acid hydrolysis, 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization, infrared spectroscopy, HPLC, GC-MS, and NMR spectroscopy, identified BFZ213 as a novel mannogalactan (160 kDa). The backbone comprises 1, 3-linked mannose, 1, 2, 3-linked mannose, 1, 4linked glucuronic acid, and 1, 2, 4, 6-linked galactose, with branching at the C-4 and C-6 positions of 1, 2, 4, 6-linked galactose, including 1, 4-linked galactose, 1, 6-linked galactose, terminal galactose, and arabinose. Bioactivity studies demonstrated that BFZ213 significantly suppressed TNF-alpha and IL-1(3 secretion in LPSstimulated NCM460 cells and attenuated MAPK and NF-kappa B signaling by inhibiting the phosphorylation of p38, JNK, p65, and I kappa B alpha. These results highlight BFZ213 as a promising lead compound for IBD therapy, underscoring the therapeutic relevance of mannogalactans. |
| WOS关键词 | POLYSACCHARIDES ; METHYLATION ; MICE |
| 资助项目 | National Natural Science Foundation of China[2022YFA1303802] ; Shanghai Post-doctoral Excellence Program[82341097] ; Shanghai Post-doctoral Excellence Program[32271332] ; Shanghai Post-doctoral Excellence Program[32471328] ; High-level Innovative Research Institute[2022686] ; Department of Science and Technology of Guangdong Province[2021B0909050003] ; Ninth Batch of Innovative Scientific Research Team Projects[CXTD2022012] ; Zhongshan Science and Technology Bureau |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001458680600001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316935]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Jin, Can; Ding, Kan |
| 作者单位 | 1.Nanchang Univ, Jiangxi Med Coll, Sch Pharm, Nanchang 330019, Peoples R China 2.Henan Polysaccharide Res Ctr, Zhengzhou 450046, Peoples R China 3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210029, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 6.Henan Univ Chinese Med, Henan Key Lab Chinese Med Polysaccharides & Drugs, Zhengzhou 450046, Peoples R China 7.Henan Univ Chinese Med, Acad Chinese Med Sci, Zhengzhou 450046, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, Hongchen,Lai, Zhengyang,Shi, Lexue,et al. Chemical structure and anti-inflammatory effects on intestinal epithelial cells of a novel mannogalactan purified from Typhonium giganteum Engl.[J]. CARBOHYDRATE RESEARCH,2025,552:13. |
| APA | Luo, Hongchen,Lai, Zhengyang,Shi, Lexue,Hu, Yulong,Jin, Can,&Ding, Kan.(2025).Chemical structure and anti-inflammatory effects on intestinal epithelial cells of a novel mannogalactan purified from Typhonium giganteum Engl..CARBOHYDRATE RESEARCH,552,13. |
| MLA | Luo, Hongchen,et al."Chemical structure and anti-inflammatory effects on intestinal epithelial cells of a novel mannogalactan purified from Typhonium giganteum Engl.".CARBOHYDRATE RESEARCH 552(2025):13. |
入库方式： OAI收割
来源：上海药物研究所
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