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A positive-sense single-stranded RNA virus acquired a negative-sense open reading frame through recombination

文献类型：期刊论文

作者Dong, Xuan8,9,10; Zhang, Fan8,9,10; Wang, Yiting8,9,10; Li, Cixiu7; Li, Xuan8,9,10; Zhou, Chengyan8,9,10; Wang, Guohao8,9,10; Tian, Zhongshuai6; Gao, Jing5; Zhou, Hu5
刊名PLOS PATHOGENS
出版日期2025-04-01
卷号21期号:4页码:16
ISSN号1553-7366
DOI10.1371/journal.ppat.1013015
英文摘要Although positive- and negative-sense single-stranded RNA viruses are ubiquitous in nature, there is currently no evidence of recombination or reassortment between viruses with these two major forms of genome organization. Here, we describe the discovery of brine shrimp virga-like virus 1 (BSVV1), a novel positive-sense single-stranded RNA virus with a recombinant genome structure derived from two viral phyla with differing genome organizations. The genome of BSVV1 comprises three open reading frames (ORFs). ORF1 resembles the RNA-dependent RNA polymerase of Ips virga-like virus 1 (a positive-sense RNA virus), while ORF2, transcribed in the positive orientation, is related to the glycoprotein of Hubei bunya-like virus 10 and other negative-sense RNA viruses. The predicted ORF3 was unique to BSVV1 without known homologs identified. The presence of the three protein products was verified by mass spectrometry. Notably, our analysis also revealed that BSVV1 is geographically widespread and found in brine shrimp from at least eight countries on four continents. In addition, BSVV1 was successfully cultured and proliferated to high viral loads during brine shrimp development. In sum, we provide compelling evidence of an ancient recombination event between negative- and positive-sense single-stranded RNA viruses, enriching our understanding of the evolution of genome structures in RNA viruses.
WOS关键词MOLECULAR-BIOLOGY ; SEQUENCE ; ALIGNMENT ; BUNYAVIRUS ; PEPTIDE ; REGION
资助项目National Key R&D Program of China[2023YFD2402200] ; Qingdao Municipal Natural Science Foundation[23-2-1-181-zyyd-jch] ; Taishan Scholars Program, Shandong Key R&D Program (Competitive Innovation Platform)[2024CXPT071-3] ; Central Public-interest Scientific Institution Basal Research Fund, CAFS[2023TD42] ; China Agriculture Research System[CARS-48]
WOS研究方向Microbiology ; Parasitology ; Virology
语种英语
WOS记录号WOS:001462112300001
出版者PUBLIC LIBRARY SCIENCE
源URL[http://119.78.100.183/handle/2S10ELR8/316965]  
专题新药研究国家重点实验室
通讯作者Huang, Jie; Shi, Weifeng
作者单位1.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai, Peoples R China
2.Univ Sydney, Sch Med Sci, Sydney, NSW, Australia
3.Univ Ghent, Fac Biosci Engn, Lab Aquaculture & Artemia Reference Ctr, Ghent, Belgium
4.Univ Ghent, Fac Vet Med, Lab Virol, Ghent, Belgium
5.Chinese Acad Sci, Dept Analyt Chem, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai, Peoples R China
6.Shanghai Jiao Tong Univ, Shanghai Inst Virol, Sch Med, Shanghai, Peoples R China
7.Shandong First Med Univ & Shandong Acad Med Sci, Key Lab Emerging Infect Dis Univ Shandong, Jinan, Peoples R China
8.Minist Agr & Rural Affairs, Key Lab Maricultural Organism Dis Control, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Qingdao, Peoples R China
9.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
10.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Dong, Xuan,Zhang, Fan,Wang, Yiting,et al. A positive-sense single-stranded RNA virus acquired a negative-sense open reading frame through recombination[J]. PLOS PATHOGENS,2025,21(4):16.
APA Dong, Xuan.,Zhang, Fan.,Wang, Yiting.,Li, Cixiu.,Li, Xuan.,...&Shi, Weifeng.(2025).A positive-sense single-stranded RNA virus acquired a negative-sense open reading frame through recombination.PLOS PATHOGENS,21(4),16.
MLA Dong, Xuan,et al."A positive-sense single-stranded RNA virus acquired a negative-sense open reading frame through recombination".PLOS PATHOGENS 21.4(2025):16.

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来源：上海药物研究所

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