Development and validation of an LC-MS/MS method with precolumn derivatization for the determination of dimethoxyethyl phthalate metabolites ethylene glycol methyl ether and methoxyacetic acid in rat plasma
文献类型：期刊论文
| 作者 | Cui, Xinran1,2; Wang, Yongbin1,2; Zhan, Yan2 ; Chen, Xiaoyan1,2
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2025-08-15 |
| 卷号 | 261页码:8 |
| 关键词 | LC-MS/MS Derivatization Ethylene glycol methyl ether Methoxyacetic acid Dimethoxyethyl phthalate |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2025.116847 |
| 英文摘要 | Dimethoxyethyl phthalate (DMEP) is an industrial phthalates reagent exposed to human body. Its hydrolytic metabolite ethylene glycol methyl ether (EGME) and further oxidative metabolite methoxyacetic acid (MAA) have reproductive and developmental toxicity, and the latter is more toxic than the former. However, due to the lack of sensitive and reliable analytical method, the in vivo clearance and biotransformation of EGME and MAA is poorly understood. In order to evaluate their toxicokinetic profiles, a precolumn derivatization liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of EGME and MAA in rat plasma. After a simple protein precipitation procedure, the sample was derivatized with p-toluene sulfonyl isocyanate (PTSI) and 4-bromo-N-methylbenzylamine (4-BNMA), separated by gradient elution on HSS T3 column, and detected by electrospray ionization (ESI) source in positive multiple reaction monitoring (MRM) mode. The results of the method validation showed that EGME had good linearity in the range of 2.00-400 ng/mL and MAA in the range of 5.00-1000 ng/mL. The intra-and inter-day accuracy and precision were within acceptable limits ( +/- 15 %) at all concentrations. This validated method was successfully applied to characterize the toxicokinetics of EGME and MAA in rats following a gavage administration of 50 mg/kg DMEP. |
| WOS关键词 | MASS-SPECTROMETRY ; 2-METHOXYACETIC ACID ; GAS-CHROMATOGRAPHY ; MONOMETHYL ETHER ; EXPOSURE ; IONIZATION ; TOXICITY ; ESTERS ; WATER |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001463079800001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316986]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | 1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, 501 Haike Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Xinran,Wang, Yongbin,Zhan, Yan,et al. Development and validation of an LC-MS/MS method with precolumn derivatization for the determination of dimethoxyethyl phthalate metabolites ethylene glycol methyl ether and methoxyacetic acid in rat plasma[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2025,261:8. |
| APA | Cui, Xinran,Wang, Yongbin,Zhan, Yan,&Chen, Xiaoyan.(2025).Development and validation of an LC-MS/MS method with precolumn derivatization for the determination of dimethoxyethyl phthalate metabolites ethylene glycol methyl ether and methoxyacetic acid in rat plasma.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,261,8. |
| MLA | Cui, Xinran,et al."Development and validation of an LC-MS/MS method with precolumn derivatization for the determination of dimethoxyethyl phthalate metabolites ethylene glycol methyl ether and methoxyacetic acid in rat plasma".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 261(2025):8. |
入库方式： OAI收割
来源：上海药物研究所
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