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Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase-2 Activity via an Energy Diversion Process

文献类型：期刊论文

作者Huang, Weijing1; Zeng, Wenhui1; Huang, Zheng1; Fang, Daqing2; Liu, Hong2; Feng, Min3; Mao, Liang4; Ye, Deju1
刊名ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
出版日期2024-06-21
卷号63期号:26
关键词Afterglow luminescence ratiometric imaging activatable probes energy diversion MMP-2
DOI10.1002/anie.202404244
文献子类Article
英文摘要Ratiometric afterglow luminescent (AGL) probes are attractive for in vivo imaging due to their high sensitivity and signal self-calibration function. However, there are currently few ratiometric AGL probes available for imaging enzymatic activity in living organisms. Here, we present an energy diversion (ED) strategy that enables the design of an enzyme-activated ratiometric AGL probe (RAG-RGD) for in vivo afterglow imaging. The ED process provides RAG-RGD with a radiative transition for an 'always on' 520-nm AGL signal (AGL(520)) and a cascade three-step energy transfer (ET) process for an 'off-on' 710-nm AGL signal (AGL(710)) in response to a specific enzyme. Using matrix metalloproteinase-2 (MMP-2) as an example, RAG-RGD shows a significant similar to 11-fold increase in AGL(710)/AGL(520) toward MMP-2. This can sensitively detect U87MG brain tumors through ratiometric afterglow imaging of MMP-2 activity, with a high signal-to-background ratio and deep imaging depth. Furthermore, by utilizing the self-calibration effect of ratiometric imaging, RAG-RGD demonstrated a strong negative correlation between the AGL(710)/AGL(520) value and the size of orthotopic U87MG tumor, enabling accurate monitoring of orthotopic glioma growth in vivo. This ED process may be applied for the design of other enzyme-activated ratiometric afterglow probes for sensitive afterglow imaging.
WOS关键词NANOPARTICLES ; DESIGN
WOS研究方向Chemistry, Multidisciplinary
语种英语
WOS记录号WOS:001229340200001
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/317004]  
专题新药研究国家重点实验室
通讯作者Feng, Min; Mao, Liang; Ye, Deju
作者单位1.Nanjing Univ, Chem & Biomed Innovat Ctr ChemBIC, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China;
2.Chinese Acad Sci, State Key Lab Drug Res, Shanghai 201203, Peoples R China;
3.Nanjing Univ, Affiliated Hosp, Nanjing Drum Tower Hosp, Dept Gen Surg,Med Sch, Nanjing 210008, Peoples R China;
4.Nanjing Univ, Chem & Biomed Innovat Ctr, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Weijing,Zeng, Wenhui,Huang, Zheng,et al. Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase-2 Activity via an Energy Diversion Process[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2024,63(26).
APA Huang, Weijing.,Zeng, Wenhui.,Huang, Zheng.,Fang, Daqing.,Liu, Hong.,...&Ye, Deju.(2024).Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase-2 Activity via an Energy Diversion Process.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,63(26).
MLA Huang, Weijing,et al."Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase-2 Activity via an Energy Diversion Process".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 63.26(2024).

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来源：上海药物研究所

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