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A One-Step Strategy for the Global Characterization of Core-Fucosylated Glycoproteome

文献类型：期刊论文

作者Wang, Yuqiu1,2; Yuan, Rui3; Liang, Bo4; Zhang, Jing5; Wen, Qin3; Chen, Hongxu3; Tian, Yinping6; Wen, Liuqing3,6,7; Zhou, Hu2,3,7,8
刊名JACS AU
出版日期2024-05-01
卷号4期号:5页码:2005-2018
关键词glycosylation glycoproteomics core-fucosylation chemoenzymatic labeling one-step strategy lung adenocarcinoma biomarker
DOI10.1021/jacsau.4c00214
文献子类Article
英文摘要Core fucosylation, a special type of N-linked glycosylation, is important in tumor proliferation, invasion, metastatic potential, and therapy resistance. However, the core-fucosylated glycoproteome has not been extensively profiled due to the low abundance and poor ionization efficiency of glycosylated peptides. Here, a one-step strategy has been described for protein core-fucosylation characterization in biological samples. Core-fucosylated peptides can be selectively labeled with a glycosylated probe, which is linked with a temperature-sensitive poly(N-isopropylacrylamide) (PNIPAM) polymer, by mutant endoglycosidase (EndoF3-D165A). The labeled probe can be further removed by wild-type endoglycosidase (EndoF3) in a traceless manner for mass spectrometry (MS) analysis. The feasibility and effectiveness of the one-step strategy are evaluated in bovine serum albumin (BSA) spiked with standard core-fucosylated peptides, H1299, and Jurkat cell lines. The one-step strategy is then employed to characterize core-fucosylated sites in human lung adenocarcinoma, resulting in the identification of 2494 core-fucosylated sites distributed on 1176 glycoproteins. Further data analysis reveals that 196 core-fucosylated sites are significantly upregulated in tumors, which may serve as potential drug development targets or diagnostic biomarkers. Together, this one-step strategy has great potential for use in global and in-depth analysis of the core-fucosylated glycoproteome to promote its mechanism research.
WOS关键词GROWTH-FACTOR RECEPTOR ; CHEMOENZYMATIC SYNTHESIS ; IDENTIFICATION ; CANCER ; FRAGMENTATION ; GLYCOSYLATION ; ACCUMULATION ; ACTIVATION ; INHIBITION ; PATHWAY
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001234591500001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/317016]  
专题新药研究国家重点实验室
通讯作者Tian, Yinping; Wen, Liuqing; Zhou, Hu
作者单位1.Fudan Univ, Eye & ENT Hosp, Dept Otolaryngol, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, State Key Lab Drug Res, Shanghai 201203, Peoples R China
3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
4.Xinxiang Cent Hosp, Dept Hematol, Xinxiang 453000, Peoples R China
5.Tongji Univ, Shanghai Pulm Hosp, Sch Med, Dept Thorac Surg, Shanghai 200433, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Based Drug Res Ctr, State Key Lab Chem Biol, Shanghai 201203, Peoples R China
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
8.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yuqiu,Yuan, Rui,Liang, Bo,et al. A One-Step Strategy for the Global Characterization of Core-Fucosylated Glycoproteome[J]. JACS AU,2024,4(5):2005-2018.
APA Wang, Yuqiu.,Yuan, Rui.,Liang, Bo.,Zhang, Jing.,Wen, Qin.,...&Zhou, Hu.(2024).A One-Step Strategy for the Global Characterization of Core-Fucosylated Glycoproteome.JACS AU,4(5),2005-2018.
MLA Wang, Yuqiu,et al."A One-Step Strategy for the Global Characterization of Core-Fucosylated Glycoproteome".JACS AU 4.5(2024):2005-2018.

入库方式： OAI收割

来源：上海药物研究所

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