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Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7

文献类型：期刊论文

作者Yang, Jun1,2,3; Liang, Kai4; Ke, Han2,3; Zhang, Yuebin5; Meng, Qian6; Gao, Lei2,3; Fan, Junping2,3; Li, Guohui5; Zhou, Hu6,7; Xiao, Junyu1,4
刊名JACS AU
出版日期2024-02-12
卷号4期号:2页码:619-634
关键词deoxynivalenol degradation Fhb7 thermal stability consensus sequence protein engineering syntheticbiology Fusarium head blight
DOI10.1021/jacsau.3c00696
文献子类Article
英文摘要In the era of global climate change, the increasingly severe Fusarium head blight (FHB) and deoxynivalenol (DON) contamination have caused economic losses and brought food and feed safety concerns. Recently, an FHB resistance gene Fhb7 coding a glutathione-S transferase (GST) to degrade DON by opening the critical toxic epoxide moiety was identified and opened a new window for wheat breeding and DON detoxification. However, the poor stability of Fhb7 and the elusiveness of the catalytic mechanism hinder its practical application. Herein, we report the first structure of Fhb7 at 2.41 angstrom and reveal a unique catalytic mechanism of epoxide opening transformation in GST family proteins. Furthermore, variants V29P and M10 showed that 5.5-fold and 266.7-fold longer half-life time than wild-type, respectively, were identified. These variants offer broad substrate scope, and the engineered biosafe Bacillus subtilis overexpressing the variants shows excellent DON degradation performance, exhibiting potential at bacterium engineering to achieve DON detoxification in the feed and biomedicine industry. This work provides a profound mechanistic insight into the enzymatic activities of Fhb7 and paves the way for further utilizing Fhb7-related enzymes in crop breeding and DON detoxification by synthetic biology.
WOS关键词FUSARIUM MYCOTOXIN DEOXYNIVALENOL ; RANDOM MUTAGENESIS LIBRARIES ; UDP-GLUCOSYLTRANSFERASE ; MEGAPRIMER PCR ; GRAMINEARUM ; BARLEY ; STRATEGIES ; RESISTANCE ; ACCURACY ; WHEAT
WOS研究方向Chemistry, Multidisciplinary
语种英语
WOS记录号WOS:001164561300001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/317023]  
专题新药研究国家重点实验室
通讯作者Xiao, Junyu; Lei, Xiaoguang
作者单位1.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
2.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn,Minist Educ,Dept, Beijing 100871, Peoples R China
3.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
4.Peking Univ, Sch Life Sci, Beijing 100871, Peoples R China
5.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Mol Modeling & Design, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China
6.Chinese Acad Sci, Shanghai Inst Mat Media, Analyt Res Ctr Organ & Biol Mol, State Key Lab Drug Res, Shanghai 201203, Peoples R China
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
8.Shenzhen Bay Lab, Inst Canc Res, Shenzhen 518107, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Jun,Liang, Kai,Ke, Han,et al. Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7[J]. JACS AU,2024,4(2):619-634.
APA Yang, Jun.,Liang, Kai.,Ke, Han.,Zhang, Yuebin.,Meng, Qian.,...&Lei, Xiaoguang.(2024).Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7.JACS AU,4(2),619-634.
MLA Yang, Jun,et al."Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7".JACS AU 4.2(2024):619-634.

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来源：上海药物研究所

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