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Comprehensive Metabolite Profiling in Single-Cell Systems via Dual-Modal MALDI-Mass Spectrometry Imaging

文献类型：期刊论文

作者Yuan, Jie7; Li, Xiafei3,4,5; Shen, Xinxin6,7; Xiong, Pei2,3,4; Zhu, Nanlin7; Ye, Yang2,3,4,5,6; Liu, Jia1,7
刊名ANALYTICAL CHEMISTRY
出版日期2025-04-16
卷号97期号:16页码:8729-8737
ISSN号0003-2700
DOI10.1021/acs.analchem.4c05480
英文摘要The development of spatial multiomics technologies, particularly matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), has revolutionized our ability to map metabolic processes at single-cell resolution. However, the current techniques face challenges in minimizing matrix interferences and achieving comprehensive metabolite detection across multiple ionization modes. In this study, we present a novel dual-modal MSI workflow that leverages the pairing of 1,5-diaminonaphthalene (DAN) and its hydrochloric salt (DAN-HCl) matrices for sequential detection in positive- and negative-ion modes, respectively. This approach significantly enhanced metabolite coverage, spanning both lipid-based and nonlipid small molecules, while eliminating the need for solvent cleaning steps. Applied to a coculture of cholangiocarcinoma (CCLP1) and hepatic stellate (LX2) cells, the workflow revealed significant metabolic distinctions, including differential accumulation of glycerolipids and energy-related metabolites, highlighting the unique metabolic profiles of each cell type. Additionally, several unidentified metabolites were detected, indicating the potential to discover novel metabolic variations. These findings establish our method as a robust tool for single-cell spatial metabolomics with broad applicability in studying complex cellular interactions and advancing both research and clinical applications.
WOS关键词1,5-DIAMINONAPHTHALENE ; FIBROBLASTS ; LIPIDS
资助项目National Natural Science Foundation of China[82373827] ; National Natural Science Foundation of China
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001468572400001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/317452]  
专题新药研究国家重点实验室
通讯作者Liu, Jia
作者单位1.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310058, Peoples R China
2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Chem Dept, Shanghai 201203, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
5.Henan Univ, Sch Pharm, Kaifeng 475004, Peoples R China
6.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China
7.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Yuan, Jie,Li, Xiafei,Shen, Xinxin,et al. Comprehensive Metabolite Profiling in Single-Cell Systems via Dual-Modal MALDI-Mass Spectrometry Imaging[J]. ANALYTICAL CHEMISTRY,2025,97(16):8729-8737.
APA Yuan, Jie.,Li, Xiafei.,Shen, Xinxin.,Xiong, Pei.,Zhu, Nanlin.,...&Liu, Jia.(2025).Comprehensive Metabolite Profiling in Single-Cell Systems via Dual-Modal MALDI-Mass Spectrometry Imaging.ANALYTICAL CHEMISTRY,97(16),8729-8737.
MLA Yuan, Jie,et al."Comprehensive Metabolite Profiling in Single-Cell Systems via Dual-Modal MALDI-Mass Spectrometry Imaging".ANALYTICAL CHEMISTRY 97.16(2025):8729-8737.

入库方式： OAI收割

来源：上海药物研究所

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