Screening and functional studies of Norrin-related nanobodies
文献类型：期刊论文
| 作者 | Xu, Yiwen1,2; Wang, Yiang2,4; Lan, Zhongyun2; Tuo, Yaxin3; Zhou, Siyu2; Zhao, Shuaiying1,2; Liu, Yunfeng1,2; Kong, Yingying2; Qiao, Huarui2; Xu, Jianfeng1 |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2025-07-01 |
| 卷号 | 231页码:9 |
| 关键词 | Norrin Nanobody Expression Purification |
| ISSN号 | 1046-5928 |
| DOI | 10.1016/j.pep.2025.106717 |
| 英文摘要 | Norrin is a crucial regulator of the Wnt/(3-catenin signaling pathway, playing a key role in retinal vascular development, blood-brain barrier maintenance, and neuroprotection. In this study, a Norrin fusion protein (Norrin-Fc) was successfully expressed and purified using an insect cell expression system. Camels were immunized with purified Norrin-Fc to generate nanobodies, which consist solely of the heavy chain variable domains of heavy chain antibodies (VHH) with a molecular weight of approximately 15 kDa. Two nanobody strains, Nb 1C4 and Nb 2B10, were identified for their specific binding to Norrin-Fc, and their binding affinities were further characterized. Flow cytometry analysis confirmed that Nb 1C4 and Nb 2B10 specifically bound to the Norrin-FZD4 fusion protein. Luciferase reporter assay results demonstrated that both nanobodies effectively disrupted LGR4-induced Wnt/(3-catenin signaling upon Norrin stimulation. This study represents the first successful development of nanobodies targeting Norrin, providing a strong foundation for the advancement of Norrin-related diagnostic tools and antibody therapeutics. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001476290300001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/317560]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Xu, Jianfeng; Dai, Yuanyuan; Geng, Yong |
| 作者单位 | 1.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China 2.Chinese Acad Sci, State Key Lab Drug Res, CAS Key Lab Receptor Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 3.Hubei Univ Med, Taihe Hosp, Dept Radiol, Shiyan, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Med Sci & Peking Union Med Coll, Natl Canc Ctr, Dept Pharm, Natl Clin Res Ctr Canc,Canc Hosp, Beijing 100021, Peoples R China 6.Chinese Acad Med Sci, Canc Hosp, Natl Canc Ctr, Natl Clin Res Ctr Canc, Langfang Campus, Langfang 065001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Yiwen,Wang, Yiang,Lan, Zhongyun,et al. Screening and functional studies of Norrin-related nanobodies[J]. PROTEIN EXPRESSION AND PURIFICATION,2025,231:9. |
| APA | Xu, Yiwen.,Wang, Yiang.,Lan, Zhongyun.,Tuo, Yaxin.,Zhou, Siyu.,...&Geng, Yong.(2025).Screening and functional studies of Norrin-related nanobodies.PROTEIN EXPRESSION AND PURIFICATION,231,9. |
| MLA | Xu, Yiwen,et al."Screening and functional studies of Norrin-related nanobodies".PROTEIN EXPRESSION AND PURIFICATION 231(2025):9. |
入库方式： OAI收割
来源：上海药物研究所
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