Cryopreservation of tea (Camellia sinensis L.) seeds and embryonic axes
文献类型:期刊论文
作者 | Kim HH ; Cha YS ; Baek HJ ; Cho EG ; Chae YA ; Engelmann F. |
刊名 | Cryo Letters
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出版日期 | 2002 |
卷号 | 23期号:4页码:209-216 |
中文摘要 | This study investigated the tolerance to desiccation and freezing of tea seeds, embryonic axes (EAs) and cotyledonary embryonic axes (CEAs, consisting of EAs with portions of cotyledons still attached). No seeds germinated after desiccation and cryopreservation. EAs extracted from seeds desiccated to 18.9% moisture content (fresh weight basis) and cryopreserved showed 20.7% survival but plantlet production from these EAs was impossible. When EAs and CEAs were extracted from seeds before being submitted to desiccation and freezing, survival of control and frozen samples was equivalent with both types of materials. However, plantlet production was significantly higher from control and cryopreserved CEAs than EAs. The maturity stage of the seeds from which CEAs were extracted had an important effect on their survival and plant production percentages, mature seeds providing better results than early mature and late mature seeds. The highest percentages of plantlet production from cryopreserved CEAs, which ranged between 75.1 and 80.4%, were achieved for EA moisture contents between 21.5 and 15.0% |
公开日期 | 2013-11-05 |
源URL | [http://ir.xtbg.org.cn/handle/353005/4752] ![]() |
专题 | 西双版纳热带植物园_文献共享 |
推荐引用方式 GB/T 7714 | Kim HH,Cha YS,Baek HJ,et al. Cryopreservation of tea (Camellia sinensis L.) seeds and embryonic axes[J]. Cryo Letters,2002,23(4):209-216. |
APA | Kim HH,Cha YS,Baek HJ,Cho EG,Chae YA,&Engelmann F..(2002).Cryopreservation of tea (Camellia sinensis L.) seeds and embryonic axes.Cryo Letters,23(4),209-216. |
MLA | Kim HH,et al."Cryopreservation of tea (Camellia sinensis L.) seeds and embryonic axes".Cryo Letters 23.4(2002):209-216. |
入库方式: OAI收割
来源:西双版纳热带植物园
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