In this work, cadmium (Cd) tolerant bacteria (Cupriavidus sp. H200) and silicon-doped ferrihydrite (SiFh) were used for the first time to remediate Cd-contaminated soil, and the remediation effects of each strategy were evaluated by comparing the different substrate materials (humic acid, hydroxyapatite and biochar). The results demonstrated that SiFh + Cupriavidus sp. H200 had the best remediation effect. Compared with the blank treatment group, SiFh + Cupriavidus sp. H200 increased the content of residual Cd in soil (1.007 mg & sdot;kg-1) and reduced the concentration of Cd in the Diffusive Gradients in Thin-films (DGT) sampling (4.55 mu g & sdot;L-1). The DGT Induced Fluxes in Sediments (DIFS) model revealed that Cupriavidus sp. H200 + remediation materials significantly inhibited the resupply capacity of Cd in the soil solid phase, diminished each of the partition coefficient, adsorption rate constant and desorption rate constant of Cd. SiFh + Cupriavidus sp. H200 significantly reduced the bioavailability of Cd to pakchoi (50.01 %) and increased or maintained the activities of sucrase, alkaline phosphatase, urease, and catalase in the soil. The potential explanation for the experimental results was that SiFh reacted with the secretion of Cupriavidus sp. H200 to form microsilicate, which reduced the mobility of Cd in soil. Moreover, Cupriavidus sp. H200 + SiFh could also reduce the bioavailability of Cd by regulating the expression of Cd transport genes in pakchoi. This study offered a novel solution for the combined mineral-biological remediation of Cd-contaminated soil.