Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis
文献类型：期刊论文
| 作者 | Shao, Zhizhuo1,2,3,4; Guo, Menglin1,2,3,4 ; Wang, Hong1,2,3,4; Gu, Wenhui2,3,4; Xie, Xiujun2,3,4; Wang, Guangce2,3,4
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| 刊名 | PLANT SCIENCE
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| 出版日期 | 2025-07-01 |
| 卷号 | 356页码:10 |
| 关键词 | Pyropia yezoensis Respiratory burst oxidase homolog Carbonic anhydrase Low C i and high light condition |
| ISSN号 | 0168-9452 |
| DOI | 10.1016/j.plantsci.2025.112497 |
| 通讯作者 | Xie, Xiujun(xjxie@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Pyropia yezoensis is an important intertidal economic macroalgae, which is periodically affected by various stresses, such as the limitation of inorganic carbon (Ci) deficiency. Under such environment, the redox homeostasis within the cells of P. yezoensis is seriously affected, and the reactive oxygen species (ROS) signal transduction system would be activated to regulate the photosynthetic activity. Therefore, how P. yezoensis manage ROS to maintain effective photosynthetic carbon fixation has aroused great interest. Here, we characterize transformants overexpressing respiratory burst oxidase homolog (Rboh), an important gene that can actively produce ROS, at the levels of cellular physiology, biochemistry, and transcriptomics. Our data indicated the expression of Rboh significantly increased, accompanied by a significant upregulated expression of alpha-type carbonic anhydrase 3 (alpha CA3) and increased extracellular carbonic anhydrase activity in the Rboh overexpressing strains. Interestingly, compared with the wild type, the photosynthetic activity of transgenic strains was significantly higher under the low Ci and high light condition, implying that the ROS signal triggered by over-expression of Rboh was involved in regulating the Ci absorption and utilization in P. yezoensis when the Ci source was limited. In summary, this study provided evidence supporting the correlation between the ROS production and the Ci utilization under stress environments in P. yezoensis. |
| WOS关键词 | CONCENTRATING MECHANISMS ; OXIDATIVE STRESS ; CLIMATE-CHANGE ; CO2 ; PHOTOSYNTHESIS ; FLUORESCENCE ; TOLERANCE ; PHYTOPLANKTON ; BICARBONATE ; RESPONSES |
| 资助项目 | Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program[2022LZGC004] ; Foundation of State Key Laboratory of Mariculture Biobreeding and Sustainable Goods[BRESG202308] ; Natural Science Foundation of Qingdao[24-4-4-zrjj-145-jch] ; Shandong Province Natural Science Foundation[ZR2024MD011] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; China Agriculture Research System of MOF and MARA[CARS-50] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001470637500001 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/201602]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Xie, Xiujun; Wang, Guangce |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao, Zhizhuo,Guo, Menglin,Wang, Hong,et al. Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis[J]. PLANT SCIENCE,2025,356:10. |
| APA | Shao, Zhizhuo,Guo, Menglin,Wang, Hong,Gu, Wenhui,Xie, Xiujun,&Wang, Guangce.(2025).Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis.PLANT SCIENCE,356,10. |
| MLA | Shao, Zhizhuo,et al."Overexpression of Rboh enhances inorganic carbon acquisition through coordinating with carbonic anhydrase in Pyropia yezoensis".PLANT SCIENCE 356(2025):10. |
入库方式： OAI收割
来源：海洋研究所
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