Genome-Wide Mining of Chitinase Diversity in the Marine Diatom Thalassiosira weissflogii and Functional Characterization of a Novel GH19 Enzyme
文献类型：期刊论文
| 作者 | Cheng, Mengzhen1,2,3 ; Li, Shuang1,2,3; Wang, Jiahui2,3; Yang, Xiaoqi2,3; Duan, Delin2,3; Shao, Zhanru2,3
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| 刊名 | MARINE DRUGS
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| 出版日期 | 2025-03-26 |
| 卷号 | 23期号:4页码:18 |
| 关键词 | beta-chitin chitinase gene family glycoside hydrolase 19 diatom enzymatic activity |
| DOI | 10.3390/md23040144 |
| 通讯作者 | Duan, Delin(dlduan@qdio.ac.cn) ; Shao, Zhanru(zrshao@qdio.ac.cn) |
| 英文摘要 | Chitin represents a globally abundant marine polymer with significant ecological and biotechnological value. beta-chitin is an important carbon fixation product of diatoms and has a greater range of applications than alpha- and gamma-chitin. However, there has been a paucity of research on the characterization of chitin-related enzymes from beta-chitin producers. In this study, we performed a genome-wide identification of 38 putative chitinase genes in Thalassiosira weissflogii, a key producer of beta-chitin. Through comprehensive analyses of phylogenetic relationships, conserved motifs, structural domains, and subcellular localization predictions, we revealed that T. weissflogii possesses evolutionarily distinct GH18 and GH19 chitinase families exhibiting unique motif and domain configurations. Subcellular localization predictions showed that most TwChis were presumed to be located in the chloroplast, with a few being present in the nucleus and extracellular. The enzymatic activity of TwChi2, a GH19 chitinase, showed that TwChi2 was a member of exochitinase (EC 3.2.1.201) with strong thermal stability (40 degrees C) and broad substrate adaptability of hydrolyzing bipolymer, 1% and 5% colloidal chitin, alpha-chitin and beta-chitin. Altogether, we analyzed the chitinase gene family and characterized a highly active exochitinase from T. weissflogii, which can catalyze the degradation of both chitin polymers and chitin oligosaccharides. The relevant results lay a foundation for the internal regulation mechanism of chitin metabolism in diatoms and provide a candidate enzyme for the green industrial preparation of high-value chitin oligosaccharides. |
| WOS关键词 | HYDROLASE FAMILY 18 ; HIPPOPHAE-RHAMNOIDES ; ALPHA-CHITIN ; BETA-CHITIN ; PURIFICATION ; GENES ; IDENTIFICATION ; PROTEINS ; CLONING |
| 资助项目 | National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS[2022205] ; Shandong Provincial Natural Science Foundation[ZR2022QC141] ; [42306142] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001475295600001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/201739]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Duan, Delin; Shao, Zhanru |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Mengzhen,Li, Shuang,Wang, Jiahui,et al. Genome-Wide Mining of Chitinase Diversity in the Marine Diatom Thalassiosira weissflogii and Functional Characterization of a Novel GH19 Enzyme[J]. MARINE DRUGS,2025,23(4):18. |
| APA | Cheng, Mengzhen,Li, Shuang,Wang, Jiahui,Yang, Xiaoqi,Duan, Delin,&Shao, Zhanru.(2025).Genome-Wide Mining of Chitinase Diversity in the Marine Diatom Thalassiosira weissflogii and Functional Characterization of a Novel GH19 Enzyme.MARINE DRUGS,23(4),18. |
| MLA | Cheng, Mengzhen,et al."Genome-Wide Mining of Chitinase Diversity in the Marine Diatom Thalassiosira weissflogii and Functional Characterization of a Novel GH19 Enzyme".MARINE DRUGS 23.4(2025):18. |
入库方式： OAI收割
来源：海洋研究所
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