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Mining Arylsulfatase from Genome-Scale Metabolic Pathways of Pseudoalteromonas sp. SR43-6 and Its Agar-Based Desulfurization Applications

文献类型：期刊论文

作者Li, Songzhi3,4; Guo, Yang3; Jiang, Hong1,2; Zhang, Huan3; Li, Jiayu2; Chen, Yanli1; Li, Jie4; Mao, Xiangzhao1,2; Wang, Minxiao3
刊名ACS OMEGA
出版日期2025-04-22
卷号10期号:17页码:18005-18016
ISSN号2470-1343
DOI10.1021/acsomega.5c01356
通讯作者Jiang, Hong(jh@ouc.edu.cn) ; Mao, Xiangzhao(xzhmao@ouc.edu.cn) ; Wang, Minxiao(wangminxiao@qdio.ac.cn)
英文摘要Arylsulfatase catalyzes the cleavage of sulfate ester bonds and plays a role in agar desulfation, thereby enhancing agar gel strength and quality. While studying the desulfurization pathway in Pseudoalteromonassp. SR43-6, a sequence encoding a potential arylsulfatase-Pseudoalteromonas Ars (Ps-Ars)-was found. The enzyme, with p-nitrophenyl sulfate as a substrate, exhibited optimal activity at 35 degrees C and pH 8.0. Its relative activity (206 U/mg) exceeded that of the recently identified arylsulfatases. Four hundred units of the enzyme removed 86.4% of sulfate groups from Gelidium amansii agar in 4 h, whereas 800 U of the enzyme removed 71.3% of sulfate groups from Gracilaria lemaneiformis agar in 8 h. After enzymatic treatment, G. amansii agar gel strength was enhanced by 32%, and a similar improvement was observed in G. lemaneiformis agar gel strength. Enzymatic agar desulfurization offers mild, quality-retaining, and environmentally friendly advantages, augmenting industrial application prospects.
WOS关键词ESCHERICHIA-COLI ; MARINE BACTERIUM ; SULFATED POLYSACCHARIDE ; BETA-AGARASE ; PURIFICATION ; GENE ; OVEREXPRESSION ; DESULFATATION ; EXPRESSION ; ENZYMES
资助项目National Natural Science Foundation of China[2023YFC2811501] ; National Key R&D Program of China[32072159] ; National Natural Science Foundation of China[ZR2024Y0054] ; Natural Science Foundation of Shandong Province[323CXTD383] ; Natural Science Foundation of Shandong Province[322QN338] ; Natural Science Foundation of Hainan Province[tsqn202312099] ; Taishan Scholar Project of Shandong Province[2023KJ041] ; Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China[202262003] ; Fundamental Research Funds for the Central Universities[2023CXGC010414] ; Major Scientific and Technological Innovation Project of Shandong Province
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001472538200001
出版者AMER CHEMICAL SOC
源URL[http://ir.qdio.ac.cn/handle/337002/201748]  
专题中国科学院海洋研究所
通讯作者Jiang, Hong; Mao, Xiangzhao; Wang, Minxiao
作者单位1.Ocean Univ China, Sanya Oceanog Inst, Sanya 572025, Peoples R China
2.Ocean Univ China, Coll Food Sci & Engn, State Key Lab Marine Food Proc & Safety Control, Qingdao 266404, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266000, Peoples R China
4.Qingdao Univ Technol, Coll Environm & Municipal Engn, Qingdao 266520, Peoples R China
推荐引用方式
GB/T 7714		 Li, Songzhi,Guo, Yang,Jiang, Hong,et al. Mining Arylsulfatase from Genome-Scale Metabolic Pathways of Pseudoalteromonas sp. SR43-6 and Its Agar-Based Desulfurization Applications[J]. ACS OMEGA,2025,10(17):18005-18016.
APA Li, Songzhi.,Guo, Yang.,Jiang, Hong.,Zhang, Huan.,Li, Jiayu.,...&Wang, Minxiao.(2025).Mining Arylsulfatase from Genome-Scale Metabolic Pathways of Pseudoalteromonas sp. SR43-6 and Its Agar-Based Desulfurization Applications.ACS OMEGA,10(17),18005-18016.
MLA Li, Songzhi,et al."Mining Arylsulfatase from Genome-Scale Metabolic Pathways of Pseudoalteromonas sp. SR43-6 and Its Agar-Based Desulfurization Applications".ACS OMEGA 10.17(2025):18005-18016.

入库方式： OAI收割

来源：海洋研究所

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