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Genomic insights into ORs gene family of G protein-coupled receptors expansion driving omnivorous feeding in Spotted knifejaw (Oplegnathus punctatus)

文献类型：期刊论文

作者Wu, Yanduo2,4,5; Li, Jun3,4,5; Xiao, Zhizhong3,4,5; Li, Wensheng1; Xiao, Yongshuang3,4,5
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2025-05-01
卷号309页码:25
关键词Olfactory receptor Oplegnathus punctatus Dietary habits Transcriptome analysis
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2025.142674
通讯作者Li, Jun(junli@qdio.ac.cn) ; Xiao, Yongshuang(yongshuangxiao@qdio.ac.cn)
英文摘要The Spotted knifejaw was previously considered a predominantly carnivorous species, but emerging evidence suggests that it exhibits omnivorous characteristics. However, the mechanisms underpinning its dietary remain poorly understood; Behavioral experiments have demonstrated that the fish shows preferences for both carnivorous and herbivorous foods and olfactory dysfunction was found to significantly reduced the responsiveness to food stimuli. Anatomical and histological analyses revealed a well-developed olfactory system, with increases in the number of olfactory lamellae and epithelial cells at 50 and 120 dph, which were consistent with spatio-temporal gene expression patterns. Genomic analyses identified ORs within the GPCR family, including 164 MORs, 77 TAARs, 4 V1Rs, and 7 V2Rs. Notably, a significant expansion of the delta subtype of MORs was observed suggesting a role in omnivorous adaptation. Transcriptomic WGCNA revealed the pathways related to protein digestion and absorption, pancreatic secretion, olfactory transduction, and gastric acid secretion. It is hypothesized that the expansion of TAAR13c is related to the carnivorous nature of O. punctatus. In situ hybridization confirmed the expression of key ORs in olfactory epithelial cells, sensory neurons, and intestinal endocrine cells, and functional validation of ORs using the dual luciferase assay, providing new insights into the molecular mechanisms governing omnivory in the Spotted knifejaw.
WOS关键词OLFACTORY RECEPTOR ; MULTIGENE FAMILY ; ODORANT RECEPTORS ; ATLANTIC SALMON ; EXPRESSION ; BEHAVIOR ; MAIN ; REPERTOIRE ; RESPONSES ; ALIGNMENT
资助项目National Natural Science Foundation of China[42276107] ; Natural Science Foundation of Shandong Province[ZR2024MC071] ; Shandong Province Key RD Program[2023LZGCQY001] ; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences[COMS2020Q05] ; National Key Research and Development Program[2022-36]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001463864500001
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/201587]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Li, Jun; Xiao, Yongshuang
作者单位1.Laizhou Mingbo Aquat Prod Co Ltd, Yantai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
5.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Yanduo,Li, Jun,Xiao, Zhizhong,et al. Genomic insights into ORs gene family of G protein-coupled receptors expansion driving omnivorous feeding in Spotted knifejaw (Oplegnathus punctatus)[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2025,309:25.
APA Wu, Yanduo,Li, Jun,Xiao, Zhizhong,Li, Wensheng,&Xiao, Yongshuang.(2025).Genomic insights into ORs gene family of G protein-coupled receptors expansion driving omnivorous feeding in Spotted knifejaw (Oplegnathus punctatus).INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,309,25.
MLA Wu, Yanduo,et al."Genomic insights into ORs gene family of G protein-coupled receptors expansion driving omnivorous feeding in Spotted knifejaw (Oplegnathus punctatus)".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 309(2025):25.

入库方式： OAI收割

来源：海洋研究所

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