The dynamic features and microbial mechanism of nitrogen transformation in small greenhouse farming system of Pacific white shrimp, Litopenaeus vannamei
文献类型：期刊论文
| 作者 | Huang, Yong1,3,4 ; Liu, Mei2; Jiang, Keyong3,4; Zhong, Chen2; Xu, Kefeng2; Wang, Baojie3,4; Wang, Lei1,3,4
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| 刊名 | AQUACULTURE
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| 出版日期 | 2025-07-15 |
| 卷号 | 605页码:14 |
| 关键词 | Small greenhouse farming system Denitrification Nitrification Surface sediment Suspended particulate matter |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2025.742557 |
| 通讯作者 | Wang, Baojie(wangbaojie@qdio.ac.cn) ; Wang, Lei(leiwang@qdio.ac.cn) |
| 英文摘要 | The proliferation of small greenhouse farms for Litopenaeus vannamei cultivation in China represents an emerging dominant paradigm in shrimp aquaculture. However, inorganic nitrogen accumulation in water during cultivation significantly impacts yield and economic benefits. In this study, water quality analysis, particle size analysis, quantitative Real time-PCR and metagenomics were employed to investigate the main nitrogen transformations, the characteristics of surface sediment (SS) and suspended particulate matter (SP), the attached microbial communities, and the response characteristics of the main functional genes in the small greenhouse. The results indicated that the changes in inorganic nitrogen concentration in the small greenhouse exhibited phased characteristics. The increase in the abundance of ammonia-oxidizing and nitrite-oxidizing bacteria reduced the ammonia and nitrite concentrations in water to less than 1 mg/L during the turbid-turned stage. During the maturity stage, the abundance of key denitrification functional genes (nirS and nirK) both increased to more than 1 x 108 copies/g, promoting the removal of nitrate from the water. SS and SP were mainly composed of silt and clay, providing abundant ecological niches for nitrifying and denitrifying bacteria. The metagenomic results indicated that Defluviimonas, Rhodohalobacter, Nitrosomonas and Nitrobacter were the main nitrifying and denitrifying functional microorganisms in SS and SP. These findings demonstrated that SS-and SP-mediated nitrification and denitrification processes served as primary mechanisms for inorganic nitrogen transformation and removal in small greenhouse farming systems. This study provided critical insights into microbial nitrogen transformation processes in small greenhouse shrimp aquaculture, establishing a theoretical framework for enhanced water quality management and aquaculture practices. |
| WOS关键词 | BACTERIA ; REMOVAL ; GROWTH |
| 资助项目 | Shandong Provincial Natural Science Foundation[ZR2023MC096] ; Creation of New Germplasm of Shrimp and Oyster and Construction of a New Model for Efficient and Clean Production[2024BBSA] ; Key R&D Project of Shandong Province[2023TZXD047] ; Project of Factory-scale Shrimp Healthy Aquaculture Digitization Key Technology and Recirculating Aquaculture Systems/Unmanned Workshop Assessment[DC6129I202120002] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001471496100001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/201617]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Baojie; Wang, Lei |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Municipal Engn Res Ctr Aquat Biol Qual Evaluat & A, Marine Sci Res Inst Shandong Prov, Shandong Prov Key Lab Dis Control Mariculture, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Yong,Liu, Mei,Jiang, Keyong,et al. The dynamic features and microbial mechanism of nitrogen transformation in small greenhouse farming system of Pacific white shrimp, Litopenaeus vannamei[J]. AQUACULTURE,2025,605:14. |
| APA | Huang, Yong.,Liu, Mei.,Jiang, Keyong.,Zhong, Chen.,Xu, Kefeng.,...&Wang, Lei.(2025).The dynamic features and microbial mechanism of nitrogen transformation in small greenhouse farming system of Pacific white shrimp, Litopenaeus vannamei.AQUACULTURE,605,14. |
| MLA | Huang, Yong,et al."The dynamic features and microbial mechanism of nitrogen transformation in small greenhouse farming system of Pacific white shrimp, Litopenaeus vannamei".AQUACULTURE 605(2025):14. |
入库方式： OAI收割
来源：海洋研究所
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