First Genomic Survey of Pleurocryptella fimbriata Provides Preliminary Insights into Genome Characteristics and Evolution of a Deep-Sea Parasitic Isopod
文献类型：期刊论文
| 作者 | Wang, Aiyang1,2,3,4 ; Hui, Min1,4; Sha, Zhongli1,2,3,4
|
| 刊名 | DIVERSITY-BASEL
 |
| 出版日期 | 2025-04-20 |
| 卷号 | 17期号:4页码:15 |
| 关键词 | Bopyridae
mitochondrial genome
parasitism
|
| DOI | 10.3390/d17040297 |
| 通讯作者 | Hui, Min(minhui@qdio.ac.cn) ; Sha, Zhongli(shazl@qdio.ac.cn) |
| 英文摘要 | Genomic adaptations of parasitic crustaceans in deep-sea extreme environments are poorly understood. This study presents the first genome survey of Pleurocryptella fimbriata, a bopyrid isopod parasitizing deep-sea squat lobsters, using Illumina sequencing. The genome size was estimated to be 1.06 Gb via a K-mer analysis, smaller than its free-living relatives. The repeat content and heterozygosity were 66.31% and 1.14%, respectively, indicating a complex genome. The draft genome assembly yielded 0.93 Gb of scaffolds with an N50 length of 989 bp, and a complete mitochondrial genome of 14,711 bp was obtained. Phylogenetic analyses of 13 mitochondrial protein-coding genes confirmed the monophyly of Bopyridae, supporting Pleurocryptella as the most primitive genus within the group and the key role of deep sea in the origin and diversification of bopyrids. A mitochondrial gene variation analysis identified NAD2 and NAD4 as promising DNA markers for a population genetic study of P. fimbriata. Twenty-four positively selected sites across COX1, NAD2, and NAD4 genes in P. fimbriata explained the genetic basis of its adaptive evolution at the mitochondrial level. These findings provide valuable genomic resources for deep-sea parasitic crustaceans and establish a foundation for further high-quality genome assembly and adaptive mechanism studies of P. fimbriata. |
| WOS关键词 | DETECTING POSITIVE SELECTION ; HYDROTHERMAL-VENT ; MITOCHONDRIAL GENOME ; DECAPODA ; CRUSTACEA ; SEQUENCE ; ADAPTATIONS ; BOPYRIDAE ; INFERENCE ; PLATFORM |
| 资助项目 | National Science Foundation of China (NSFC) for Distinguished Young Scholars ; NSFC Innovative Group Grant[42221005] ; NSFC[42376143] ; NSFC for Distinguished Young Scholars[42025603] |
| WOS研究方向 | Biodiversity & Conservation ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001474957800001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/201707]  |
| 专题 | 海洋研究所_海洋生物分类与系统演化实验室 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Hui, Min; Sha, Zhongli |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Dept Marine Organism Taxon & Phylogeny, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Aiyang,Hui, Min,Sha, Zhongli. First Genomic Survey of Pleurocryptella fimbriata Provides Preliminary Insights into Genome Characteristics and Evolution of a Deep-Sea Parasitic Isopod[J]. DIVERSITY-BASEL,2025,17(4):15. |
| APA | Wang, Aiyang,Hui, Min,&Sha, Zhongli.(2025).First Genomic Survey of Pleurocryptella fimbriata Provides Preliminary Insights into Genome Characteristics and Evolution of a Deep-Sea Parasitic Isopod.DIVERSITY-BASEL,17(4),15. |
| MLA | Wang, Aiyang,et al."First Genomic Survey of Pleurocryptella fimbriata Provides Preliminary Insights into Genome Characteristics and Evolution of a Deep-Sea Parasitic Isopod".DIVERSITY-BASEL 17.4(2025):15. |
入库方式： OAI收割
来源：海洋研究所
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