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A polysaccharide from Gynostemma pentaphyllum: structure characterization and anti-insulin resistance potential through Galectin-3 modulation

文献类型：期刊论文

作者Ren, Mingwang5,6; Sun, Guoqing1,5; Ding, Xiaoyu5; Yu, Xiaolin5,6; He, Li5; Xiao, Shiji4,5,6; Dong, Minjian4,5,6; Yang, Jianwen3,5; Ding, Kan2,5; Sun, Chengxin4,5,6
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2025-05-01
卷号310页码:8
关键词Gynostemma pentaphyllum Polysaccharides Structural characterization Insulin resistance Galectin-3
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2025.143618
通讯作者Yang, Jianwen(450699491@qq.com) ; Ding, Kan(dingkan@simm.ac.cn) ; Sun, Chengxin(suncx926@nenu.edu.cn)
英文摘要Polysaccharides are among the key bioactive components of Gynostemma pentaphyllum (Thunb.) Makino. In this study, a novel polysaccharide fraction, GPPA1-1, was isolated from G. pentaphyllum and purified using DEAESepharose Fast Flow, Sephadex G-75, and Sepharose CL-6B column chromatography. GPPA1-1 was determined to have a molecular weight of 3.7 x 104 Da and was composed of mannose (Man), glucuronic acid (GlcA), rhamnose (Rha), glucose (Glc), galactose (Gal), and arabinose (Ara) in a molar ratio of 3.88:3.97:21.77:7.02:45.65:17.71. Comprehensive structural characterization was conducted using Congo red staining, FT-IR spectroscopy, Methylation analysis, and NMR analysis. The backbone of GPPA1-1 was identified as comprising-*4)-alpha-Galp-(1-*,-*4)-alpha-Galp-(3,1-*,-*2)-alpha-Rhap-(4,1-*, and-*2)-alpha-Rhap-(1-*, with branch chains containing alpha-Araf-(1-*,-*5)-alpha-Araf-(1-*,-*3)-alpha-Galp-(1-*, and-alpha-GlcA-(1-*. Biological assays demonstrated that GPPA1-1 effectively alleviates insulin resistance by inhibiting Galectin-3. This activity was confirmed through various experiments, including Galectin-3-mediated hemagglutination, Western blotting, CETSA, and glucose uptake assays. These findings highlight the potential of GPPA1-1 as a promising therapeutic agent for managing insulin resistance.
WOS关键词OXIDATIVE STRESS ; ANTIOXIDANT ACTIVITIES
资助项目National Natural Sciences Foundation of China[31960204] ; Science and Technology Department of Guizhou Province[QKHZC [2020] 4Y211] ; Science and Technology Department of Guizhou Province[QKHJC [2020] 1Y402] ; Science and Technology Department of Guizhou Province[QSKH2019003-005] ; Guizhou Key Laboratory of Modern Traditional Chinese Medicine Creation[Qian Ke He Platform ZSYS [2025] 019] ; Zunyi Medical University[F-465] ; Zunyi Medical University[F-930]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001484683700003
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/317875]  
专题中国科学院上海药物研究所
通讯作者Yang, Jianwen; Ding, Kan; Sun, Chengxin
作者单位1.Third Peoples Hosp Dalian, Dept Pharm, Dalian 116091, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China
3.Zunyi Med Univ, Affiliated Hosp, Dept Pharm, Zunyi 563003, Peoples R China
4.Zunyi Med Univ, Key Lab Basic Pharmacol, Minist Educ, Zunyi 563006, Peoples R China
5.Zunyi Med Univ, Sch Pharm, Zunyi 563006, Peoples R China
6.Zunyi Med Univ, Guizhou Key Lab Modern Tradit Chinese Med Creat, Zunyi 563006, Peoples R China
推荐引用方式
GB/T 7714		 Ren, Mingwang,Sun, Guoqing,Ding, Xiaoyu,et al. A polysaccharide from Gynostemma pentaphyllum: structure characterization and anti-insulin resistance potential through Galectin-3 modulation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2025,310:8.
APA Ren, Mingwang.,Sun, Guoqing.,Ding, Xiaoyu.,Yu, Xiaolin.,He, Li.,...&Sun, Chengxin.(2025).A polysaccharide from Gynostemma pentaphyllum: structure characterization and anti-insulin resistance potential through Galectin-3 modulation.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,310,8.
MLA Ren, Mingwang,et al."A polysaccharide from Gynostemma pentaphyllum: structure characterization and anti-insulin resistance potential through Galectin-3 modulation".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 310(2025):8.

入库方式： OAI收割

来源：上海药物研究所

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