Integrated strategy by combining preparative HSCCC with HPLC-UV-QDa for efficient isolation of specific friedelane-type triterpenoids from Salix tetrasperma
文献类型:期刊论文
作者 | Wu, Pei-Qian2; Fang, Chu-Hong1; Zhou, Bin1,2; Yue, Jian-Min1,2![]() |
刊名 | JOURNAL OF CHROMATOGRAPHY A
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出版日期 | 2025-07-19 |
卷号 | 1753页码:13 |
关键词 | Salix tetrasperma Friedelane-type triterpenoids HSCCC Multiple detectors Antiproliferative activity |
ISSN号 | 0021-9673 |
DOI | 10.1016/j.chroma.2025.465912 |
英文摘要 | Salicin derivatives, the most abundant secondary metabolites in Salix tetrasperma Roxb, interfere with the purification of the trace triterpenoids in the plant, which are characterized by minimal or no UV absorption. Herein, a modified isolation approach, integrating preparative high-speed countercurrent chromatography with preparative HPLC with UV/vis and ACQUITY QDa detectors, was applied to remove salicin derivatives and purify the minor triterpenoids of S. tetrasperma. Application of the strategy led to a successful isolation of 11 new and highly oxidized friedelane-type triterpenoids, salixtetrones A-K (1-11), and one rare 29-nor-friedelane-type triterpenoid salixtetrone L (12) from the ethyl acetate extract of S. tetrasperma barks. This is, to the best of our knowledge, the first report on the friedelane-type triterpenoids from species of Salix L. The structures of all compounds were elucidated mainly by conventional spectroscopic and spectrometric data analyses, coupled with single-crystal X-ray diffraction. Compounds 1 and 8 showed moderate antiproliferative activity against the A549 cell line. |
WOS关键词 | SOLVENT-SYSTEM ; CHROMATOGRAPHY |
资助项目 | National Natural Science Foundation of China[82322064] ; Shanghai Institute of Materia Medica of CAS[SIMM0120231002] ; Youth Innovation Promotion Association of CAS[2022283] ; Chinese Academy of Sci-ences |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
WOS记录号 | WOS:001484837400001 |
出版者 | ELSEVIER |
源URL | [http://119.78.100.183/handle/2S10ELR8/317884] ![]() |
专题 | 新药研究国家重点实验室 |
通讯作者 | Zhou, Bin; Yue, Jian-Min |
作者单位 | 1.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China 2.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Wu, Pei-Qian,Fang, Chu-Hong,Zhou, Bin,et al. Integrated strategy by combining preparative HSCCC with HPLC-UV-QDa for efficient isolation of specific friedelane-type triterpenoids from Salix tetrasperma[J]. JOURNAL OF CHROMATOGRAPHY A,2025,1753:13. |
APA | Wu, Pei-Qian,Fang, Chu-Hong,Zhou, Bin,&Yue, Jian-Min.(2025).Integrated strategy by combining preparative HSCCC with HPLC-UV-QDa for efficient isolation of specific friedelane-type triterpenoids from Salix tetrasperma.JOURNAL OF CHROMATOGRAPHY A,1753,13. |
MLA | Wu, Pei-Qian,et al."Integrated strategy by combining preparative HSCCC with HPLC-UV-QDa for efficient isolation of specific friedelane-type triterpenoids from Salix tetrasperma".JOURNAL OF CHROMATOGRAPHY A 1753(2025):13. |
入库方式: OAI收割
来源:上海药物研究所
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