Characterization of the Biological Effect Mediated by Mycobacterial Kinase PknG on Protein Phosphorylation and Acetylation
文献类型:期刊论文
作者 | Xie, Shuyu2,3,4; Liu, Tianqi4; Zhang, Luoxi1; Yang, Yang1; Tang, Yanqing4; Ye, Tong2,3; Sun, Yewen4; Li, Yi3,4; Shen, Shiyang3,4; Tan, Minjia1,2,3,4![]() |
刊名 | ACS OMEGA
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出版日期 | 2025-05-21 |
卷号 | 10期号:21页码:21662-21673 |
ISSN号 | 2470-1343 |
DOI | 10.1021/acsomega.5c01032 |
英文摘要 | Tuberculosis (TB) remains a significant global public health challenge, with post-translational modifications (PTMs) in mycobacteria being integral to physiological processes, pathogenesis, and drug resistance mechanisms. Protein kinase G (PknG), the only reported secreted kinase in mycobacteria, is associated with virulence, immune evasion, and drug resistance. Nevertheless, the comprehensive range of proteins and phosphorylation modifications regulated by PknG in mycobacteria has not yet been fully elucidated. This study conducted a systematic investigation into the dynamic alterations in the proteome and phosphoproteome of mycobacteria following the overexpression of PknG. A comprehensive analysis identified 3836 proteins and 467 phosphorylation sites, revealing the regulatory role of MsPknG in energy metabolism pathways and binding processes. Additionally, the study highlighted the interplay between protein phosphorylation and lysine acetylation, noting a reduction in lysine acetylation that is linked to carbon metabolism and various energy-related pathways in the MsPknG overexpression strains. The role of MsPknG in growth, infection, and the secretion of the inflammatory factor was further investigated, providing new insights into phosphorylation functions within mycobacteria and potential clinical targets for tuberculosis treatment. The study additionally provides an innovative perspective on the interplay between post-translational modifications in mycobacteria. |
WOS关键词 | TUBERCULOSIS ; MECHANISM ; SURVIVAL ; METABOLISM ; GLYOXYLATE ; VIRULENCE ; GROWTH |
资助项目 | Sanofi[32322048] ; Sanofi[32471497] ; National Natural Science Foundation of China[22QA1411100] ; Shanghai Rising-Star Program[2023YFA1800400] ; Shanghai Rising-Star Program[2023YFA1800403] ; National Key Research and Development Program of China[CXTD2024006] ; Zhongshan Science and Technology Bureau[22XD1420900] ; Program of Shanghai Academic Research Leader[2022QNRC001] ; Young Elite Scientists Sponsorship Program by CAST[CAS2021276] ; Youth Innovation Promotion Association[019B090904008] ; Sanofi Scholarship Program, Guangdong High-level New R&D Institute, China[2021B0909050003] ; Guangdong High-level Innovative Research Institute, China ; Innovative Research Team of high-level local universities in Shanghai |
WOS研究方向 | Chemistry |
语种 | 英语 |
WOS记录号 | WOS:001493305900001 |
出版者 | AMER CHEMICAL SOC |
源URL | [http://119.78.100.183/handle/2S10ELR8/317941] ![]() |
专题 | 新药研究国家重点实验室 |
通讯作者 | Tan, Minjia; Xu, Jun-Yu |
作者单位 | 1.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Peoples R China 2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Jiangsu, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Shanghai 201203, Guangdong, Peoples R China |
推荐引用方式 GB/T 7714 | Xie, Shuyu,Liu, Tianqi,Zhang, Luoxi,et al. Characterization of the Biological Effect Mediated by Mycobacterial Kinase PknG on Protein Phosphorylation and Acetylation[J]. ACS OMEGA,2025,10(21):21662-21673. |
APA | Xie, Shuyu.,Liu, Tianqi.,Zhang, Luoxi.,Yang, Yang.,Tang, Yanqing.,...&Xu, Jun-Yu.(2025).Characterization of the Biological Effect Mediated by Mycobacterial Kinase PknG on Protein Phosphorylation and Acetylation.ACS OMEGA,10(21),21662-21673. |
MLA | Xie, Shuyu,et al."Characterization of the Biological Effect Mediated by Mycobacterial Kinase PknG on Protein Phosphorylation and Acetylation".ACS OMEGA 10.21(2025):21662-21673. |
入库方式: OAI收割
来源:上海药物研究所
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