中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A BODIPY-tagged trivalent glycocluster for receptor-targeting fluorescence imaging of live cells

文献类型:期刊论文

作者Guo, Chen3,5,6; Si, Fang-Yu5,6; Wang, Chen-Han4,5,6; Wang, Ning5,6; Hu, Xi-Le5,6; James, Tony D.1,2; Li, Jia3; Wang, Chengyun5,6; He, Xiao-Peng4,5,6
刊名CHEMICAL SCIENCE
出版日期2025-06-25
卷号16期号:25页码:11481-11486
ISSN号2041-6520
DOI10.1039/d4sc08472a
英文摘要Multivalent glycoclusters have been extensively used as a targeting agent for drug delivery. However, tools capable of investigating their dynamic interactions with a target receptor remain elusive. Here, we synthesized fluorescently-tagged galactoclusters for the fluorescence imaging of cells that overly express the asialoglycoprotein receptor (ASGPr). A trivalent galactoside was synthesized, to which a boron dipyrromethene (BODIPY) dye was conjugated. The resulting fluorescent glycocluster was used for the targeted fluorescence imaging of liver cancer cells with a high ASGPr expression level. The trivalent probe was also demonstrated to be applicable for super-resolution imaging of ASGPr-mediated ligand endocytosis and the dynamic intracellular translocation to the lysosomes. As such, this study provides a suitable chemical tool for the study of receptor dynamics using fluorescently tagged glycoclusters.
WOS关键词ASIALOGLYCOPROTEIN RECEPTOR ; ENDOCYTOSIS ; PROBE
资助项目National Natural Science Foundation of China[92253306] ; National Natural Science Foundation of China[82130099] ; National Natural Science Foundation of China[22477030] ; Natural National Science Foundation of China (NSFC)[24DX1400200] ; Science and Technology Commission of Shanghai Municipality[23490711600] ; International Cooperation Program of Shanghai Science and Technology[222201717003] ; Fundamental Research Funds for the Central Universities[B16017] ; Programme of Introducing Talents of Discipline to Universities[24ZR1415400] ; National Natural Science Foundation of Shanghai Science and Technology[QNKJ2024010] ; Shanghai Oriental Talents youth Program[23XHYD-20] ; Shanghai Xuhui District Hospital Local Cooperation Project[KF 2402] ; Open Funding Project of the State Key Laboratory of Fine Chemicals, Dalian University of Technology ; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University[2023-MEKLLC-MS/ZD-00*] ; Ministry of Education Key Laboratory on signaling Regulation and Targeting Therapy of Liver Cancer (Naval Medical University)[SYS202205] ; Shandong Laboratory Program ; University of Bath[2020ZD01] ; Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University ; Research Center of Analysis and Test of East China University of Science and Technology
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001495509000001
出版者ROYAL SOC CHEMISTRY
源URL[http://119.78.100.183/handle/2S10ELR8/317971]  
专题新药研究国家重点实验室
通讯作者James, Tony D.; Li, Jia; Wang, Chengyun; He, Xiao-Peng
作者单位1.Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China
2.Univ Bath, Dept Chem, Bath BA2 7AY, England
3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Natl Ctr Liver Canc, Eastern Hepatobiliary Surg Hosp, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China
5.East China Univ Sci & Technol, Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn,Feringa N, 130 Meilong Rd, Shanghai 200237, Peoples R China
6.East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China
推荐引用方式
GB/T 7714
Guo, Chen,Si, Fang-Yu,Wang, Chen-Han,et al. A BODIPY-tagged trivalent glycocluster for receptor-targeting fluorescence imaging of live cells[J]. CHEMICAL SCIENCE,2025,16(25):11481-11486.
APA Guo, Chen.,Si, Fang-Yu.,Wang, Chen-Han.,Wang, Ning.,Hu, Xi-Le.,...&He, Xiao-Peng.(2025).A BODIPY-tagged trivalent glycocluster for receptor-targeting fluorescence imaging of live cells.CHEMICAL SCIENCE,16(25),11481-11486.
MLA Guo, Chen,et al."A BODIPY-tagged trivalent glycocluster for receptor-targeting fluorescence imaging of live cells".CHEMICAL SCIENCE 16.25(2025):11481-11486.

入库方式: OAI收割

来源:上海药物研究所

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