A BODIPY-tagged trivalent glycocluster for receptor-targeting fluorescence imaging of live cells
文献类型:期刊论文
作者 | Guo, Chen3,5,6; Si, Fang-Yu5,6; Wang, Chen-Han4,5,6; Wang, Ning5,6; Hu, Xi-Le5,6; James, Tony D.1,2; Li, Jia3![]() |
刊名 | CHEMICAL SCIENCE
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出版日期 | 2025-06-25 |
卷号 | 16期号:25页码:11481-11486 |
ISSN号 | 2041-6520 |
DOI | 10.1039/d4sc08472a |
英文摘要 | Multivalent glycoclusters have been extensively used as a targeting agent for drug delivery. However, tools capable of investigating their dynamic interactions with a target receptor remain elusive. Here, we synthesized fluorescently-tagged galactoclusters for the fluorescence imaging of cells that overly express the asialoglycoprotein receptor (ASGPr). A trivalent galactoside was synthesized, to which a boron dipyrromethene (BODIPY) dye was conjugated. The resulting fluorescent glycocluster was used for the targeted fluorescence imaging of liver cancer cells with a high ASGPr expression level. The trivalent probe was also demonstrated to be applicable for super-resolution imaging of ASGPr-mediated ligand endocytosis and the dynamic intracellular translocation to the lysosomes. As such, this study provides a suitable chemical tool for the study of receptor dynamics using fluorescently tagged glycoclusters. |
WOS关键词 | ASIALOGLYCOPROTEIN RECEPTOR ; ENDOCYTOSIS ; PROBE |
资助项目 | National Natural Science Foundation of China[92253306] ; National Natural Science Foundation of China[82130099] ; National Natural Science Foundation of China[22477030] ; Natural National Science Foundation of China (NSFC)[24DX1400200] ; Science and Technology Commission of Shanghai Municipality[23490711600] ; International Cooperation Program of Shanghai Science and Technology[222201717003] ; Fundamental Research Funds for the Central Universities[B16017] ; Programme of Introducing Talents of Discipline to Universities[24ZR1415400] ; National Natural Science Foundation of Shanghai Science and Technology[QNKJ2024010] ; Shanghai Oriental Talents youth Program[23XHYD-20] ; Shanghai Xuhui District Hospital Local Cooperation Project[KF 2402] ; Open Funding Project of the State Key Laboratory of Fine Chemicals, Dalian University of Technology ; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University[2023-MEKLLC-MS/ZD-00*] ; Ministry of Education Key Laboratory on signaling Regulation and Targeting Therapy of Liver Cancer (Naval Medical University)[SYS202205] ; Shandong Laboratory Program ; University of Bath[2020ZD01] ; Open Research Fund of the School of Chemistry and Chemical Engineering, Henan Normal University ; Research Center of Analysis and Test of East China University of Science and Technology |
WOS研究方向 | Chemistry |
语种 | 英语 |
WOS记录号 | WOS:001495509000001 |
出版者 | ROYAL SOC CHEMISTRY |
源URL | [http://119.78.100.183/handle/2S10ELR8/317971] ![]() |
专题 | 新药研究国家重点实验室 |
通讯作者 | James, Tony D.; Li, Jia; Wang, Chengyun; He, Xiao-Peng |
作者单位 | 1.Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China 2.Univ Bath, Dept Chem, Bath BA2 7AY, England 3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Natl Ctr Liver Canc, Eastern Hepatobiliary Surg Hosp, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China 5.East China Univ Sci & Technol, Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn,Feringa N, 130 Meilong Rd, Shanghai 200237, Peoples R China 6.East China Univ Sci & Technol, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China |
推荐引用方式 GB/T 7714 | Guo, Chen,Si, Fang-Yu,Wang, Chen-Han,et al. A BODIPY-tagged trivalent glycocluster for receptor-targeting fluorescence imaging of live cells[J]. CHEMICAL SCIENCE,2025,16(25):11481-11486. |
APA | Guo, Chen.,Si, Fang-Yu.,Wang, Chen-Han.,Wang, Ning.,Hu, Xi-Le.,...&He, Xiao-Peng.(2025).A BODIPY-tagged trivalent glycocluster for receptor-targeting fluorescence imaging of live cells.CHEMICAL SCIENCE,16(25),11481-11486. |
MLA | Guo, Chen,et al."A BODIPY-tagged trivalent glycocluster for receptor-targeting fluorescence imaging of live cells".CHEMICAL SCIENCE 16.25(2025):11481-11486. |
入库方式: OAI收割
来源:上海药物研究所
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