E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnesses
文献类型：期刊论文
| 作者 | Zhang F(张帆)1,2,3,4 ; Zheng L(郑璐)2,3,4; Wu Y(武亿)2,3,4; Ding QH(丁奇寒)2,3,4; Lv SQ(吕守芹)2,3,4; Lv DY(吕东媛)2,3,4; Long M(龙勉)2,3,4
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| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
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| 出版日期 | 2025-06-23 |
| 卷号 | 82期号:1页码:18 |
| 关键词 | Embryonic stem cells Definitive endodermal differentiation E-cadherin Substrate stiffness YAP |
| ISSN号 | 1420-682X |
| DOI | 10.1007/s00018-025-05776-9 |
| 通讯作者 | Lu, Dongyuan(lvdongy@imech.ac.cn) ; Long, Mian(mlong@imech.ac.cn) |
| 英文摘要 | Intercellular adhesion is crucial in regulating stemness maintenance and differentiation initiation of embryonic stem cells (ESCs), which is also cooperated with extracellular mechanical microenvironment. Here an in vitro model was used to elucidate the effects of E-cadherin complexes on definitive endoderm (DE)-directed differentiation of hESCs (H1 cells), when the cells were seeded on polyacrylamide hydrogels with varied stiffnesses. Results indicated that stiff substrate increased the proportion of H1 cells differentiating into DE cells and intercellular E-cadherin expression was reduced with progressive stages at same stiffness, presenting a negative correlation of E-cadherin expression with differentiating progress or substrate stiffness. Blocking E-cadherin enhanced the productivity of differentiated cells and promoted the disassembly of intercellular adhesions by translocating YAP into nuclei, which was positively correlated with GATA6 and CXCR4 expressions in a stiffness-dependent manner. This work provided an insight into understanding the roles of E-cadherin-related intercellular adhesion and substrate stiffness in DE-directed differentiation of hESCs. |
| 分类号 | 二类/Q1 |
| WOS关键词 | CELL-MIGRATION ; PROLIFERATION ; ADHESION ; PLATFORM ; CDH1 ; YAP |
| 资助项目 | National Key Research and Development Program of China[2021YFA0719302] ; National Natural Science Foundation of China grants[32130061] ; National Natural Science Foundation of China grants[32371376] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001513968700003 |
| 资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation of China grants |
| 其他责任者 | 吕东媛，龙勉 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/101850]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 1.Shandong Univ, Cheeloo Coll Med, Sch Basic Med Sci, Med Morphol Teaching Lab, Jinan 250012, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China; 3.Chinese Acad Sci, Inst Mech, Key Lab Micrograv, Beijing 100190, Peoples R China; 4.Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang F,Zheng L,Wu Y,et al. E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnesses[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2025,82(1):18. |
| APA | 张帆.,郑璐.,武亿.,丁奇寒.,吕守芹.,...&龙勉.(2025).E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnesses.CELLULAR AND MOLECULAR LIFE SCIENCES,82(1),18. |
| MLA | 张帆,et al."E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnesses".CELLULAR AND MOLECULAR LIFE SCIENCES 82.1(2025):18. |
入库方式： OAI收割
来源：力学研究所
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