Comparative assessment of the Sikun 2000 sequencing platform for whole genome sequencing
文献类型：期刊论文
| 作者 | Cai, Keya3,4; Li, Sibin3,4; Pan, Meng2,5; Lu, Hui2,3,5; Wang, Liang4; Fang, Shengquan1; Gou, Lingzhi4; Tang, Jiang4; Kong, Yan3,5; Zhao, Li4 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2025-05-30 |
| 卷号 | 15期号:1页码:8 |
| 关键词 | Whole genome sequencing Sikun 2000 Variation detection |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-025-04170-6 |
| 通讯作者 | Zhao, Li(zhaoli@sikun.com) ; Ren, Yongyong(yongyong.ren@sjtu.edu.cn) |
| 英文摘要 | DNA sequencing technology has significantly advanced over the past five decades, particularly with the introduction of next-generation sequencing (NGS) platforms like Illumina's NovaSeq. In October 2023, Sikun introduced the Sikun 2000, a desktop NGS platform designed for rapid, cost-effective sequencing with up to 200 Gb of data per run. This study is the first to evaluate the performance of the Sikun 2000 in whole genome sequencing (WGS) and compares it with the Illumina NovaSeq 6000 and NovaSeq X platforms using five well-characterized human Genomes in a Bottle dataset. Results show that the Sikun 2000 performs competitively in variant detection, particularly excelling in single nucleotide variant accuracy. It also demonstrated a higher sequencing depth and lower proportion of low-quality reads than the NovaSeq platforms. However, its performance in insertion-deletion (Indel) detection was slightly lower than that of NovaSeq platforms. Overall, the Sikun 2000 is a qualified sequencing platform for WGS, and further evaluation in clinical and research applications is required as data continues to accumulate. |
| WOS关键词 | MAP |
| 资助项目 | Science and Technology Commission of Shanghai Municipality[2024YFC3505900] ; Science and Technology Commission of Shanghai Municipality[2024YFC3505901] ; National Key R&D Program of China[23JS1400700] ; National Key R&D Program of China[24JS2840200] ; Science and Technology Commission of Shanghai Municipality[CSTB2024TIAD-STX0006] ; Science and Technology Innovation Key R&D Program of Chongqing[2021KJZX0063] ; Key Science and Technology Project of Zhengzhou ; Neil Shen's SJTU Medical Research Fund |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001499638000027 |
| 出版者 | NATURE PORTFOLIO |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/318210]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhao, Li; Ren, Yongyong |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western M, Dept Gastroenterol, Shanghai 200437, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Dept Bioinformat & Biostat, Shanghai 200240, Peoples R China 3.Shanghai Inst Mat Med, Inst Bioinformat, Shanghai 201203, Peoples R China 4.Sikun Life Sci Co Ltd, Zhengzhou 450016, Peoples R China 5.Shanghai Jiao Tong Univ, SJTU Yale Joint Ctr Biostat & Data Sci, Tech Ctr Digital Med, Natl Ctr Translat Med, Shanghai 200240, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Keya,Li, Sibin,Pan, Meng,et al. Comparative assessment of the Sikun 2000 sequencing platform for whole genome sequencing[J]. SCIENTIFIC REPORTS,2025,15(1):8. |
| APA | Cai, Keya.,Li, Sibin.,Pan, Meng.,Lu, Hui.,Wang, Liang.,...&Ren, Yongyong.(2025).Comparative assessment of the Sikun 2000 sequencing platform for whole genome sequencing.SCIENTIFIC REPORTS,15(1),8. |
| MLA | Cai, Keya,et al."Comparative assessment of the Sikun 2000 sequencing platform for whole genome sequencing".SCIENTIFIC REPORTS 15.1(2025):8. |
入库方式： OAI收割
来源：上海药物研究所
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