Cell-Free Expression of HPV16 Minor Capsid Protein L2 and Its Interaction with S100A10
文献类型：期刊论文
| 作者 | Jiang, Wenqi1,2; Wu, Lian1,2; Shen, Xiangchun2; Fu, Qingshan Bill1,2 |
| 刊名 | PROTEIN AND PEPTIDE LETTERS
 |
| 出版日期 | 2025-05-21 |
| 页码 | 12 |
| 关键词 | HPV16 minor capsid protein L2 purification cell-free protein synthesis interaction |
| ISSN号 | 0929-8665 |
| DOI | 10.2174/0109298665390494250513110604 |
| 通讯作者 | Shen, Xiangchun(fuqingshan@simm.ac.cn) ; Fu, Qingshan Bill() |
| 英文摘要 | Background Human papillomavirus type 16 (HPV16) is implicated in various malignancies. The virus enters host cells through endocytosis, during which the minor capsid protein L2 interacts with the S100A10 subunit of the annexin A2 heterotetramer (A2t) on the host cell membrane. This interaction is critical for facilitating HPV entry and subsequent infection of human cells. Therefore, examining the interaction between the L2 protein and S100A10 is crucial for advancing our understanding of the mechanisms by which HPV16 infiltrates cells.Objective The cell-free expression (CFE) system was investigated for L2 purification. The structure of L2 was characterized and its interaction with S100A10 was explored.Methods The L2 protein was expressed using a CFE expression system, and its expression was verified via Western blotting. L2 was further purified through size-exclusion chromatography (SEC), and its structural features were preliminarily assessed using transmission electron microscopy (TEM) and circular dichroism (CD) spectroscopy. Additionally, surface plasmon resonance (SPR) was employed to analyze the interaction between L2 and S100A10.Results Western blotting confirmed the successful expression of L2. TEM and CD provided preliminary structural observations of L2, and SPR measurements yielded precise kinetic parameters for the interaction between L2 and S100A10.Conclusion In this study, we successfully expressed the HPV16 L2 protein using a cell-free protein expression system. Preliminary structural analysis using TEM and CD revealed key structural features of L2. Furthermore, SPR analysis provided detailed kinetic parameters for its interaction with S100A10. These findings provide more details on understanding L2's structural features, with broader implications for antipathogen studies. |
| WOS关键词 | HUMAN-PAPILLOMAVIRUS TYPE-16 ; OPEN READING FRAME ; ANNEXIN A2 ; VIRUS ; ENTRY ; L1 ; IDENTIFICATION |
| 资助项目 | National Natural Science Foundation of China[32271320] ; Creative Research Group of Zhongshan City (Lingnan Pharmaceutical Research and Innovation team)[CXTD2022011] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001507309400001 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/318395]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Shen, Xiangchun; Fu, Qingshan Bill |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Zhongshan 528400, Peoples R China 2.Guizhou Med Univ, Sch Pharmaceut Sci, State Key Lab Discovery & Utilizat Funct Component, Guiyang 561113, Guizhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Wenqi,Wu, Lian,Shen, Xiangchun,et al. Cell-Free Expression of HPV16 Minor Capsid Protein L2 and Its Interaction with S100A10[J]. PROTEIN AND PEPTIDE LETTERS,2025:12. |
| APA | Jiang, Wenqi,Wu, Lian,Shen, Xiangchun,&Fu, Qingshan Bill.(2025).Cell-Free Expression of HPV16 Minor Capsid Protein L2 and Its Interaction with S100A10.PROTEIN AND PEPTIDE LETTERS,12. |
| MLA | Jiang, Wenqi,et al."Cell-Free Expression of HPV16 Minor Capsid Protein L2 and Its Interaction with S100A10".PROTEIN AND PEPTIDE LETTERS (2025):12. |
入库方式： OAI收割
来源：上海药物研究所
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