Screening macrocyclic peptide libraries by yeast display allows control of selection process and affinity ranking
文献类型：期刊论文
| 作者 | Linciano, Sara7; Mazzocato, Ylenia7; Romanyuk, Zhanna7; Vascon, Filippo6; Farrera-Soler, Lluc5; Will, Edward5; Xing, Yuyu4; Chen, Shiyu4; Kumada, Yoichi3; Simeoni, Marta1,2 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2025-06-25 |
| 卷号 | 16期号:1页码:23 |
| DOI | 10.1038/s41467-025-60907-x |
| 通讯作者 | Angelini, Alessandro(alessandro.angelini@unive.it) |
| 英文摘要 | Macrocyclic peptides represent an attractive drug modality due to their favourable properties and amenability to in vitro evolution techniques such as phage or mRNA display. Although very powerful, these technologies are not without limitations. In this work, we address some of their drawbacks by developing a yeast display-based strategy to generate, screen and characterise structurally diverse disulfide-cyclised peptides. The use of quantitative flow cytometry enables real-time monitoring of the screening of millions of individual macrocyclic peptides, leading to the identification of ligands with good binding properties to five different protein targets. X-ray analysis of a selected ligand in complex with its target reveals optimal shape complementarity and extensive surface interaction, explaining its exquisite affinity and selectivity. The yeast display-based approach described here offers a facile, quantitative and cost-effective alternative to rapidly and efficiently discover and characterise genetically encoded macrocyclic peptide ligands with sufficiently good binding properties against therapeutically relevant targets. |
| WOS关键词 | BOWMAN-BIRK INHIBITOR ; ALPHA-CHYMOTRYPSIN ; SURFACE DISPLAY ; TRYPSIN-INHIBITOR ; CRYSTAL-STRUCTURE ; PHAGE SELECTION ; DISCOVERY ; COMPLEX ; LIGAND ; SCAFFOLDS |
| 资助项目 | National Recovery and Resilience Plan and the European Union-Next Generation EU, Mission 4, Component 2,[CUP B93D21010860004] ; National Centre for Gene Therapy and Drugs based on RNA Technology', Spoke 8 'Platforms for RNA/DNA delivery', |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001517178700014 |
| 出版者 | NATURE PORTFOLIO |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/318672]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Angelini, Alessandro |
| 作者单位 | 1.Ca Bottacin, European Ctr Living Technol ECLT, Dorsoduro 3911,Calle Crosera, I-30123 Venice, Italy 2.Ca Foscari Univ Venice, Dept Environm Sci Informat & Stat, Via Torino 155, I-30172 Venice, Italy 3.Kyoto Inst Technol, Dept Funct Chem & Engn, 1 Matsugasaki Hashikami Cho,Sakyo Ku, Kyoto 6060951, Japan 4.Chinese Acad Sci, Shanghai Inst Mat Med, Biotech Drug Res Ctr, Shanghai 201203, Peoples R China 5.Ecole Polytech Fed Lausanne EPFL, Inst Chem Sci & Engn, Sch Basic Sci, CH-1015 Lausanne, Switzerland 6.Univ Padua, Dept Biol, Viale G Colombo 3, Padua 35131, Italy 7.Ca Foscari Univ Venice, Dept Mol Sci & Nanosyst, Via Torino 155, I-30172 Venice, Italy |
| 推荐引用方式 GB/T 7714 | Linciano, Sara,Mazzocato, Ylenia,Romanyuk, Zhanna,et al. Screening macrocyclic peptide libraries by yeast display allows control of selection process and affinity ranking[J]. NATURE COMMUNICATIONS,2025,16(1):23. |
| APA | Linciano, Sara.,Mazzocato, Ylenia.,Romanyuk, Zhanna.,Vascon, Filippo.,Farrera-Soler, Lluc.,...&Angelini, Alessandro.(2025).Screening macrocyclic peptide libraries by yeast display allows control of selection process and affinity ranking.NATURE COMMUNICATIONS,16(1),23. |
| MLA | Linciano, Sara,et al."Screening macrocyclic peptide libraries by yeast display allows control of selection process and affinity ranking".NATURE COMMUNICATIONS 16.1(2025):23. |
入库方式： OAI收割
来源：上海药物研究所
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