Construction of colorimetric/fluorescent dual-signal probes for the detection of β-galactosidase in senescent cells
文献类型：期刊论文
| 作者 | Dong, Lei3,6; Sun, Lu-Lu5; Li, Wen-Jia2,5; Xing, Jing6; Tan, Mingchao6; Han, Yanan3; Han, Hai-Hao2,4,5; Niu, Li-Ya1; Zhang, Zhong3 |
| 刊名 | DYES AND PIGMENTS
 |
| 出版日期 | 2025-11-01 |
| 卷号 | 242页码:8 |
| 关键词 | beta-Galactosidase Fluorescence sensing Cell senescence |
| ISSN号 | 0143-7208 |
| DOI | 10.1016/j.dyepig.2025.112972 |
| 通讯作者 | Han, Hai-Hao(hanhaihao@simm.ac.cn) ; Niu, Li-Ya(niuly@bnu.edu.cn) ; Zhang, Zhong(nasonia@163.com) |
| 英文摘要 | The sensitive and selective detection of beta-galactosidase (beta-Gal) activity and spatial distribution holds critical importance for elucidating the molecular mechanisms underlying cellular senescence and associated pathologies. Herein, we present ratiometric probes (1 and 2) integrating dual signals of colorimetric and red fluorescence for precise beta-Gal detection in solution and senescent cells. Strategic introduction of chlorine on a tricyanofuran (TCF)-based fluorophore (1) enabled enzyme-responsive absorption shifts from 420 nm to 575 nm in absorption accompanied by remarkable red fluorescence enhancement (>80-fold at 622 nm). These red-shifted photophysical properties were attributed to the enhanced electron-donating ability from beta-Gal-released phenolate residue (1-O), which is stabilized through the incorporation of chlorine into the molecular structure. Probe 1 demonstrated exceptional analytical performance with detection limits of 1.2 x 10(-4) U mL(-1) (absorption) and 7.4 x 10(-5) U mL(-1) (fluorescence), outperforming conventional probes in sensitivity and selectivity. Therefore, 1 was allowed to track the endogenous beta-Gal in senescent A549 cells with a long sensing period (24 h). This work establishes a framework for designing multimodal optical probes through rational structural improvement, offering broad applicability in senescence research, diagnostic assay development, and therapeutic evaluation. |
| 资助项目 | Provincial Natural Science Foundation[ZR2023QB248] ; Taishan Scholars Program[tsqn202312305] ; Young Elite Scientists Sponsorship Program by Chinese Chemical Society ; Fundamental Research Projects of Science & Technology Innovation and development Plan in Yantai City[2023JCYJ059] ; Key R&D Program of Shandong[2024CXPT028] ; Science and Technology Support Plan for Youth Innovation in Universities of Shandong Province[2024KJG029] ; Shandong Laboratory Program[SYS202205] ; Intelligence Assistance Xinjiang Innovation and Talent Expansion Project |
| WOS研究方向 | Chemistry ; Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001520883300001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/318690]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Han, Hai-Hao; Niu, Li-Ya; Zhang, Zhong |
| 作者单位 | 1.Beijing Normal Univ, Inst Key Lab Radiopharmaceut, Coll Chem, Minist Educ, Beijing 100875, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Shandong Second Med Univ, Sch Life Sci & Technol, Weifang 261053, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Mol Imaging Ctr, Stake Key Lab Chem Biol, Shanghai 201203, Peoples R China 5.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Peoples R China 6.Shandong Second Med Univ, Sch Pharm, Weifang 261053, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Lei,Sun, Lu-Lu,Li, Wen-Jia,et al. Construction of colorimetric/fluorescent dual-signal probes for the detection of β-galactosidase in senescent cells[J]. DYES AND PIGMENTS,2025,242:8. |
| APA | Dong, Lei.,Sun, Lu-Lu.,Li, Wen-Jia.,Xing, Jing.,Tan, Mingchao.,...&Zhang, Zhong.(2025).Construction of colorimetric/fluorescent dual-signal probes for the detection of β-galactosidase in senescent cells.DYES AND PIGMENTS,242,8. |
| MLA | Dong, Lei,et al."Construction of colorimetric/fluorescent dual-signal probes for the detection of β-galactosidase in senescent cells".DYES AND PIGMENTS 242(2025):8. |
入库方式： OAI收割
来源：上海药物研究所
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