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Androgens alleviate the depression-like phenotype in female mice by inhibiting AVPR1a in the hippocampal brain region

文献类型：期刊论文

作者Ren, Shimin3,4; Wang, Xian3,4; Huang, Xueying2; Chen, Liyang1; Zhang, Bing2; Li, Yang3,4; Huang, Xin5
刊名MOLECULAR MEDICINE
出版日期2025-05-29
卷号31期号:1页码:15
关键词Androgen receptors AVPR1a Female Depression DHEA Hippocampus
ISSN号1076-1551
DOI10.1186/s10020-025-01272-9
英文摘要BackgroundThe prevalence of depression in women is approximately twice that in men. Differences in androgens levels between men and women, due to gonadal differences, may be associated with the development of depression, although the underlying mechanisms are not well understood.MethodsWe evaluated the depressive phenotypes of female mice following low-dose androgen treatment using a variety of behavioral and in vivo electrophysiological experiments. The mRNA profile of hippocampal tissues from female mice treated with dehydroepiandrosterone (DHEA) was constructed through RNA sequencing (RNA-seq). GO and KEGG pathway analyses were performed on the differentially expressed genes. The expression changes of candidate differential genes were verified in hippocampal tissues by quantitative real-time PCR and western blotting. Moreover, the mechanism of action of the DHEA-regulated differential gene (Avpr1a), which is involved in the neuroactive ligand-receptor interaction pathway, was determined in vitro.ResultsChronic DHEA treatment resulted in a distinct antidepressant phenotype and significantly enhanced neuronal excitability of the ventral hippocampal region of female mice. RNA-seq identified the crucial differentially expressed gene, Avpr1a. In vitro experiments showed that DHEA reduced levels of the AVP system. Additionally, ChIP-PCR experiments revealed that Avpr1a directly targets androgen receptor (AR). Cell function experiments demonstrated that DHEA can inhibit AVPR1a expression through AR in a dose-dependent manner, and this effect can be reversed by the androgen receptor antagonist (Flutamide).ConclusionAndrogens (DHEA) exert antidepressant effects by inhibiting the binding of Avpr1a to AR. The Avpr1a gene may serve as a new target for the treatment of depression in women.
WOS关键词CENTRAL-NERVOUS-SYSTEM ; NEUROACTIVE STEROIDS ; MAJOR DEPRESSION ; TESTOSTERONE ; VASOPRESSIN ; ESTROGEN ; DEHYDROEPIANDROSTERONE ; WOMEN ; GENE ; RAT
资助项目National Natural Science Foundation ; OE Biotech Co., Ltd. (Shanghai, China)
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology ; Research & Experimental Medicine
语种英语
WOS记录号WOS:001498086100001
出版者SPRINGER
源URL[http://119.78.100.183/handle/2S10ELR8/318195]  
专题新药研究国家重点实验室
通讯作者Zhang, Bing; Li, Yang; Huang, Xin
作者单位1.Southeast Univ, Sch Publ Hlth, Key Lab Environm Med Engn & Educ Minist, Nanjing 210009, Jiangsu, Peoples R China
2.Tongji Univ, Shanghai Matern & Infant Hosp 1, Shanghai Inst Maternal Fetal Med & Gynecol Oncol, Clin & Translat Res Ctr,Shanghai Key Lab Maternal, Shanghai 201204, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Jiangsu, Peoples R China
5.Shanghai Jiao Tong Univ, Xinhua Hosp, Sch Med, Reprod Med Ctr, Shanghai 200082, Peoples R China
推荐引用方式
GB/T 7714		 Ren, Shimin,Wang, Xian,Huang, Xueying,et al. Androgens alleviate the depression-like phenotype in female mice by inhibiting AVPR1a in the hippocampal brain region[J]. MOLECULAR MEDICINE,2025,31(1):15.
APA Ren, Shimin.,Wang, Xian.,Huang, Xueying.,Chen, Liyang.,Zhang, Bing.,...&Huang, Xin.(2025).Androgens alleviate the depression-like phenotype in female mice by inhibiting AVPR1a in the hippocampal brain region.MOLECULAR MEDICINE,31(1),15.
MLA Ren, Shimin,et al."Androgens alleviate the depression-like phenotype in female mice by inhibiting AVPR1a in the hippocampal brain region".MOLECULAR MEDICINE 31.1(2025):15.

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来源：上海药物研究所

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