Qingfeiyin decoction in idiopathic pulmonary fibrosis: Lung-targeted components and PI3K-AKT modulation
文献类型：期刊论文
| 作者 | Liu, Yongqi2,3; Song, Luyao1; Chen, Shiyi1; Li, Jiajia1; Peng, Xionghua1; Sun, Jianhua1,2 ; Tian, Ying2; Huang, Chenggang1; Gong, Li-kun1,2,3; Chen, Jing1
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2025-07-24 |
| 卷号 | 351页码:14 |
| 关键词 | Idiopathic pulmonary fibrosis Qingfeiyin Baicalin Oroxylin a Network pharmacology Molecular docking Molecular dynamic simulation |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2025.120071 |
| 英文摘要 | Ethnopharmacological relevance: Qingfeiyin decoction (QFY), a well-documented traditional Chinese medicine formula, is used to treat a variety of lung diseases. However, the effect of QFY on idiopathic pulmonary fibrosis (IPF) is still unclear. Aim of the study: This study aimed to investigate the effect of QFY on IPF, identify its bioactive components, and reveal the possible mechanism. Materials and methods: A bleomycin-induced pulmonary fibrosis mouse model was established to evaluate the anti-IPF effects of QFY. The bioactive ingredients in QFY were identified by HPLC-MS, following which their in vitro effects were examined by the cell models of epithelial-to-mesenchymal transition, fibroblast-tomyofibroblast transition and macrophage polarization. Network pharmacology and clinical dataset (GSE110147) were integrated to predict the possible mechanism of the bioactive ingredients, which were verified by in vitro experiments, molecular docking and molecular dynamics simulation. Results: QFY demonstrated dose-dependent amelioration of IPF pathological phenotypes. Oroxylin A (OA), Geniposide (GDS), Baicalin (BCL), and Genipin-1-gentiobioside (GG) were identified with significant lung tissue enrichment, where OA and BCL exhibited obvious improvement in EMT, FMT, and macrophage polarization experiments. Network pharmacology and bioinformatics analyses revealed the association of OA/GDS/BCL/GG with the PI3K-AKT signaling pathway, which were confirmed by their effects on the migration and apoptosis of A549 and HFL1 cells. Concurrently, molecular docking and molecular dynamics simulations demonstrated strong binding affinities of BCL and OA for p110 alpha and p110 gamma subunits of PI3K. Conclusions: Our work demonstrates the potential of QFY in the treatment of IPF, which might due to the bioactive ingredients in the lung affecting the PI3K signaling pathway. |
| WOS关键词 | TRADITIONAL CHINESE MEDICINE ; NETWORK PHARMACOLOGY ; RESPONSES |
| 资助项目 | Shanghai Committee of Science and Technology, China[21S21901800] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001509191800004 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/318422]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Huang, Chenggang; Gong, Li-kun; Chen, Jing |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 3.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yongqi,Song, Luyao,Chen, Shiyi,et al. Qingfeiyin decoction in idiopathic pulmonary fibrosis: Lung-targeted components and PI3K-AKT modulation[J]. JOURNAL OF ETHNOPHARMACOLOGY,2025,351:14. |
| APA | Liu, Yongqi.,Song, Luyao.,Chen, Shiyi.,Li, Jiajia.,Peng, Xionghua.,...&Chen, Jing.(2025).Qingfeiyin decoction in idiopathic pulmonary fibrosis: Lung-targeted components and PI3K-AKT modulation.JOURNAL OF ETHNOPHARMACOLOGY,351,14. |
| MLA | Liu, Yongqi,et al."Qingfeiyin decoction in idiopathic pulmonary fibrosis: Lung-targeted components and PI3K-AKT modulation".JOURNAL OF ETHNOPHARMACOLOGY 351(2025):14. |
入库方式： OAI收割
来源：上海药物研究所
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