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  2. 上海药物研究所
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  4. 新药研究国家重点实验室
Nonlinear Imaging Detection of Organ Fibrosis in Minute Samples for Early Stage Utilizing Dual-Channel Two-Photon and Second-Harmonic Excitation

文献类型：期刊论文

作者Yu, Bo-Song10; Cheng, Qing-Di8,9; Liu, Yi-Zhou6,7; Zhang, Rui8; Li, Da-Wei5; Wang, Ai-Min3,4; Feng, Li-Shuang2,10; Jia, Xiao1,9
刊名BIOSENSORS-BASEL
出版日期2025-06-04
卷号15期号:6页码:16
关键词nonlinear imaging two-photon excitation second harmonic generation fibrosis
DOI10.3390/bios15060357
英文摘要Histopathological staining remains the fibrosis diagnostic gold standard yet suffers from staining artifacts and variability. Nonlinear optical techniques (e.g., spontaneous fluorescence, Second Harmonic Generation) enhance accuracy but struggle with rapid trace-level detection of fibrosis. To address these limitations, a dual-channel nonlinear optical imaging system with excitation wavelengths at 780 nm and 820 nm was developed, enabling simultaneous spontaneous fluorescence and second-harmonic generation imaging through grid localization. This study applies dual-modality nonlinear imaging to achieve label-free, high-resolution visualization of pulmonary and renal fibrosis at the ECM microstructure scale. Through leveraging this system, it is demonstrated that collagen can be rapidly detected via spontaneous fluorescence at 780 nm, whereas the spatial distribution of collagen fibrils is precisely mapped using Second Harmonic Generation at 820 nm. This approach allows for the rapid and sensitive detection of trace fibrosis in a 5-day unilateral ureteral obstruction mouse model. Additionally, we identify that the elastic fibers, which can also be visualized, provide a foundation for staging diagnosis and delivering accurate and quantitative data for pathological studies and analysis. The research findings underscore the potential of this dual-channel nonlinear optical imaging system as a powerful tool for rapid, precise, and noninvasive fibrosis detection and staging.
WOS关键词FLUORESCENCE ; GENERATION ; DIAGNOSIS ; COLLAGEN
资助项目National Natural Science Foundation of China ; Zhongshan Municipal Bureau of Science and Technology[CXTD2024007] ; High-level Innovative Research Institute[2021B0909050003] ; [62475008] ; [62305186]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation
语种英语
WOS记录号WOS:001516119300001
出版者MDPI
源URL[http://119.78.100.183/handle/2S10ELR8/318477]  
专题新药研究国家重点实验室
通讯作者Feng, Li-Shuang; Jia, Xiao
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
2.Beihang Univ, Key Lab Precis Optomechatron Technol, Minist Educ, Beijing 100191, Peoples R China
3.Peking Univ, Sch Elect, State Key Lab Adv Opt Commun Syst & Networks, Beijing 100871, Peoples R China
4.Peking Univ, Sch Elect, Beijing 100871, Peoples R China
5.Peking Univ, Coll Future Technol, Beijing 100871, Peoples R China
6.Shandong Univ, Key Lab Laser & Infrared Syst, Minist Educ, Qingdao 266237, Peoples R China
7.Shandong Univ, Sch Informat Sci & Engn, Shandong Prov Key Lab Laser Technol & Applicat, Qingdao 266237, Peoples R China
8.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Kidney Transplantat, Guangzhou 510630, Peoples R China
9.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Zhongshan 528400, Peoples R China
10.Beihang Univ, Sch Instrumentat & Optoelect Engn, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Bo-Song,Cheng, Qing-Di,Liu, Yi-Zhou,et al. Nonlinear Imaging Detection of Organ Fibrosis in Minute Samples for Early Stage Utilizing Dual-Channel Two-Photon and Second-Harmonic Excitation[J]. BIOSENSORS-BASEL,2025,15(6):16.
APA Yu, Bo-Song.,Cheng, Qing-Di.,Liu, Yi-Zhou.,Zhang, Rui.,Li, Da-Wei.,...&Jia, Xiao.(2025).Nonlinear Imaging Detection of Organ Fibrosis in Minute Samples for Early Stage Utilizing Dual-Channel Two-Photon and Second-Harmonic Excitation.BIOSENSORS-BASEL,15(6),16.
MLA Yu, Bo-Song,et al."Nonlinear Imaging Detection of Organ Fibrosis in Minute Samples for Early Stage Utilizing Dual-Channel Two-Photon and Second-Harmonic Excitation".BIOSENSORS-BASEL 15.6(2025):16.

入库方式： OAI收割

来源：上海药物研究所

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