Integrative Proteomics and Histone PTMomics Study on Anti-TNBC Effect of JIB-04
文献类型：期刊论文
| 作者 | Tao, Shengna6,7; Guo, Jingli5,6; Zhang, Jingdan3,4; Zhang, Mingya6; Wang, Zhe2; Zhang, Jun5,6; Li, Mingjun1; Liu, Bin7; Yuan, Jian2; Tan, Minjia3,4,5,6
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| 刊名 | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
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| 出版日期 | 2025-07-02 |
| 页码 | 12 |
| 关键词 | Proteomics histone PTMomics epigenetic inhibitor JIB-04 TNBC |
| ISSN号 | 1044-0305 |
| DOI | 10.1021/jasms.5c00022 |
| 英文摘要 | The incidence of malignant breast cancer is increasing, and triple-negative breast cancer (TNBC) is particularly challenging due to its poor prognosis and limited response to conventional treatments. Recently, epigenetic inhibitors have emerged as promising therapeutic strategies for TNBC. JIB-04, that targeted histone lysine demethylases (KDMs), was reported to show anticancer activities against TNBC. However, the molecular mechanism underlying the antitumor effect of JIB-04 remains elusive. Herein, we integrated histone PTMomics and quantitative proteomics to comprehensively characterize histone PTM site changes and signaling pathway alterations. Under JIB-04 treatment, a total of 14 significantly altered histone PTM sites were identified, including previously reported sites (H3K9me2, H3K9me3, and H3K36me3) and two newly reported sites (H3K79me2 and H3K9ac). Proteomic analysis quantified 904 differentially expressed proteins, including 495 upregulated and 409 downregulated proteins. Pathway enrichment analysis suggested that JIB-04 significantly affected the DNA damage response. Further biological evidence demonstrated that JIB-04 modulated DNA damage response, cell cycle arrest, and apoptosis. In summary, our study provides new mechanistic insights into the anticancer activity of JIB-04 and offers new options for the treatment of TNBC. |
| WOS关键词 | CANCER ; PHOSPHORYLATION ; CHALLENGES ; THERAPY ; SUV39H2 ; CELLS |
| 资助项目 | National Natural Science Foundation of China[2024YFC2815903] ; National Key R&D Program of China[22XD1420900] ; Program of Shanghai Academic Research Leader[32090032] ; National Natural Science Foundation of China |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:001521559600001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/318678]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Tan, Minjia; Zhao, Wensi |
| 作者单位 | 1.Tongji Univ, Shanghai Pulm Hosp, Dept Thorac Surg, Sch Med, Shanghai 200433, Peoples R China 2.Tongji Univ, Shanghai East Hosp, State Key Lab Cardiol & Res Ctr Translat Med, Sch Med, Shanghai 200120, Peoples R China 3.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 4.Southern Med Univ, Sch Pharmaceut Sci, Guangzhou 510515, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 7.Jiangsu Ocean Univ, Coll Pharm, Jiangsu Key Lab Marine Pharmaceut Cpd Screening, Lianyungang 222005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, Shengna,Guo, Jingli,Zhang, Jingdan,et al. Integrative Proteomics and Histone PTMomics Study on Anti-TNBC Effect of JIB-04[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2025:12. |
| APA | Tao, Shengna.,Guo, Jingli.,Zhang, Jingdan.,Zhang, Mingya.,Wang, Zhe.,...&Zhao, Wensi.(2025).Integrative Proteomics and Histone PTMomics Study on Anti-TNBC Effect of JIB-04.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,12. |
| MLA | Tao, Shengna,et al."Integrative Proteomics and Histone PTMomics Study on Anti-TNBC Effect of JIB-04".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY (2025):12. |
入库方式： OAI收割
来源：上海药物研究所
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