中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 力学研究所
  3. 中国科学院力学研究所
  4. 国家微重力实验室
Integrin Tension Represents the Directed Differentiation of Mesenchymal Stem Cells via Mechanoadaptation

文献类型：期刊论文

作者Tian DW(田大伟)2,3,4; Yue, Danyang4; Zhu JC(朱婧辰)1,2,3; Zhang XY(张晓宇)1,2,3; Gong, Mingliang2,3,4; Long M(龙勉)1,2,3; Zhang, Yan1,2,3; Pan, Jun4
刊名ACS NANO
出版日期2025-07-07
卷号19期号:27页码:24850-24864
关键词integrin tension probe mechanical signaling F-actin lamin A
ISSN号1936-0851
DOI10.1021/acsnano.5c03069
通讯作者Zhang, Yan(zhangyan@imech.ac.cn) ; Pan, Jun(panj@cqu.edu.cn)
英文摘要The mechanism controlling the differentiation of mesenchymal stromal cells (MSCs) to the target cell is vital for their tissue repair. While integrins, physically connected focal adhesion proteins (FAs), cytoskeleton elements, and lamin A are involved, the quantitative roles of integrin tension and mechanoadaptation remain unknown. Here, we applied reversible shearing DNA-based tension probes (RSDTPs) to assess MSCs' integrin tension during spreading after biochemical induction to osteogenesis and chondrogenesis. The mechanical transmitters of paxillin, F-actin, and lamin A to transduce mechanical stress from integrin to the nucleus as well as differentiation of MSCs were assayed to reveal the mechanical adaptation of integrin tension. Integrin tensions of 44 and 5-18 pN were identified as the critical force for osteogenic and chondrogenic differentiations, respectively. To fit this tension difference, MSCs possessed FAs' orientation to align radially along the cell's normal axis in osteogenesis vs tangentially in chondrogenesis. Cytoskeleton F-actin was found to regulate the osteogenesis through altering the upstream integrin tension and FAs' orientation and the downstream lamin A expression but yield less influences on chondrogenesis. In turn, F-actin tended to be less modulated by lamin A and its accompanying changes in FAs and integrin tension than by biochemical induction. Lamin A favored the regulation of osteogenesis through altering the upstream integrin tension and orientations of FAs and F-actin but might play an inhibitory role in chondrogenic differentiation. This work contributes to the understanding of how integrins generate and transmit different magnitudes of forces to jointly regulate cell morphology and mechanical adaptability and to fit the differentiation fate of MSCs.
分类号一类
WOS关键词FOCAL ADHESION ; LIGAND-BINDING ; FORCE ; CYTOSKELETAL ; SHAPE ; POLARIZATION ; ORIENTATION ; FATE ; BOND
资助项目National Natural Science Foundation of China[T2394514] ; National Natural Science Foundation of China[32250017] ; NSF of China[2020CDCGSW051] ; Fundamental Research Funds for the Central Universities of China[GJJSTU20220002] ; Scientific Instrument Developing Project of the Chinese Academy of Sciences
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:001525998200001
资助机构National Natural Science Foundation of China ; NSF of China ; Fundamental Research Funds for the Central Universities of China ; Scientific Instrument Developing Project of the Chinese Academy of Sciences
其他责任者Zhang, Yan，Pan, Jun
源URL[http://dspace.imech.ac.cn/handle/311007/102026]  
专题力学研究所_国家微重力实验室
作者单位1.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China;
3.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv, Beijing 100190, Peoples R China;
4.Chongqing Univ, Coll Bioengn, State & Local Joint Engn Lab Vasc Implants, Key Lab Biorheol Sci & Technol,Minist Educ, Chongqing 400044, Peoples R China;
推荐引用方式
GB/T 7714		 Tian DW,Yue, Danyang,Zhu JC,et al. Integrin Tension Represents the Directed Differentiation of Mesenchymal Stem Cells via Mechanoadaptation[J]. ACS NANO,2025,19(27):24850-24864.
APA 田大伟.,Yue, Danyang.,朱婧辰.,张晓宇.,Gong, Mingliang.,...&Pan, Jun.(2025).Integrin Tension Represents the Directed Differentiation of Mesenchymal Stem Cells via Mechanoadaptation.ACS NANO,19(27),24850-24864.
MLA 田大伟,et al."Integrin Tension Represents the Directed Differentiation of Mesenchymal Stem Cells via Mechanoadaptation".ACS NANO 19.27(2025):24850-24864.

入库方式： OAI收割

来源：力学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。