Efficient production of the beta-ionone aroma compound from organic waste hydrolysates using an engineered Yarrowia lipolytica strain
文献类型：期刊论文
| 作者 | Chen, Shuyi4; Lu, Yanping1,5,6; Wang, Wen; Hu, Yunzi3; Wang, Jufang5; Tang, Shixing4; Lin, Carol Sze Ki2; Yang, Xiaofeng5 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2022-09-21 |
| 卷号 | 13页码:11 |
| 关键词 | metabolic engineering Yarrowia lipolytica CCD1 beta-ionone flavor and fragrance compound food and agricultural waste |
| DOI | 10.3389/fmicb.2022.960558 |
| 通讯作者 | Lin, Carol Sze Ki(carollin@cityu.edu.hk) ; Yang, Xiaofeng(biyangxf@scut.edu.cn) |
| 英文摘要 | This study demonstrates the feasibility of establishing a natural compound supply chain in a biorefinery. The process starts with the biological or chemical hydrolysis of food and agricultural waste into simple and fermentative sugars, followed by their fermentation into more complex molecules. The yeast strain, Yarrowia lipolytica, was modified by introducing high membrane affinity variants of the carotenoid cleavage dioxygenase enzyme, PhCCD1, to increase the production of the aroma compound, beta-ionone. The initial hydrolysis process converted food waste or sugarcane bagasse into nutrient-rich hydrolysates containing 78.4 g/L glucose and 8.3 g/L fructose, or 34.7 g/L glucose and 20.1 g/L xylose, respectively. During the next step, engineered Y. lipolytica strains were used to produce beta-ionone from these feedstocks. The yeast strain YLBI3120, carrying a modified PhCCD1 gene was able to produce 4 g/L of beta-ionone with a productivity of 13.9 mg/L/h from food waste hydrolysate. This is the highest yield reported for the fermentation of this compound to date. The integrated process described in this study could be scaled up to achieve economical large-scale conversion of inedible food and agricultural waste into valuable aroma compounds for a wide range of potential applications. |
| WOS关键词 | SUGARCANE BAGASSE ; FOOD WASTE ; PATHWAY ; EXPRESSION ; FERMENTATION ; TEMPERATURE ; METABOLISM ; STRATEGIES ; TERPENOIDS ; CHEMICALS |
| 资助项目 | National Key R&D Program of China ; National Natural Science Foundation of China[2018YFA0901000] ; Science and Technology Program Project of Yibin[21808068] ; [2021JC001] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000892175300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | National Key R&D Program of China ; National Natural Science Foundation of China ; Science and Technology Program Project of Yibin |
| 源URL | [http://ir.giec.ac.cn/handle/344007/37975]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Lin, Carol Sze Ki; Yang, Xiaofeng |
| 作者单位 | 1.Wuliangye Yibin Co Ltd, Technol Res Ctr, Yibin, Sichuan, Peoples R China 2.City Univ Hong Kong, Sch Energy & Environm, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou, Guangdong, Peoples R China 4.Southern Med Univ, Sch Publ Hlth, Guangdong Prov Key Lab Trop Dis Res, Guangzhou, Guangdong, Peoples R China 5.South China Univ Technol, Sch Biol & Biol Engn, Guangzhou, Guangdong, Peoples R China 6.Sichuan Yibin Wuliangye Grp Co Ltd, Postdoctoral Res Workstat, Yibin, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Shuyi,Lu, Yanping,Wang, Wen,et al. Efficient production of the beta-ionone aroma compound from organic waste hydrolysates using an engineered Yarrowia lipolytica strain[J]. FRONTIERS IN MICROBIOLOGY,2022,13:11. |
| APA | Chen, Shuyi.,Lu, Yanping.,Wang, Wen.,Hu, Yunzi.,Wang, Jufang.,...&Yang, Xiaofeng.(2022).Efficient production of the beta-ionone aroma compound from organic waste hydrolysates using an engineered Yarrowia lipolytica strain.FRONTIERS IN MICROBIOLOGY,13,11. |
| MLA | Chen, Shuyi,et al."Efficient production of the beta-ionone aroma compound from organic waste hydrolysates using an engineered Yarrowia lipolytica strain".FRONTIERS IN MICROBIOLOGY 13(2022):11. |
入库方式： OAI收割
来源：广州能源研究所
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