Zebrafish Neurotoxicity from Aphantoxins-Cyanobacterial Paralytic Shellfish Poisons (PSPs) from Aphanizomenon flos-aquae DC-1
文献类型：期刊论文
| 作者 | Zhang, Delu1,2,3; Hu, Chunxiang1; Wang, Gaohong1; Li, Dunhai1; Li, Genbao1; Liu, Yongding1 |
| 刊名 | ENVIRONMENTAL TOXICOLOGY
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 28期号:5页码:239-254 |
| 关键词 | aphantoxin apoptosis brain DNA damage ultrastructure zebrafish |
| ISSN号 | 1520-4081 |
| 通讯作者 | Liu, YD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Aphanizomenon flos-aquae (A. flos-aquae), a cyanobacterium frequently encountered in water blooms worldwide, is source of neurotoxins known as PSPs or aphantoxins that present a major threat to the environment and to human health. Although the molecular mechanism of PSP action is well known, many unresolved questions remain concerning its mechanisms of toxicity. Aphantoxins purified from a natural isolate of A. flos-aquae DC-1 were analyzed by high-performance liquid chromatography (HPLC), the major component toxins were the gonyautoxins1 and 5 (GTX1 and GTX5, 34.04% and 21.28%, respectively) and the neosaxitoxin (neoSTX, 12.77%). The LD50 of the aphantoxin preparation was determined to be 11.33 mu g/kg (7.75 mu g saxitoxin equivalents (STXeq) per kg) following intraperitoneal injection of zebrafish (Danio rerio). To address the neurotoxicology of the aphantoxin preparation, zebrafish were injected with low and high sublethal doses of A. flos-aquae DC-1 toxins 7.73 and 9.28 mu g /kg (5.3 and 6.4 mu g STXeq/kg, respectively) and brain tissues were analyzed by electron microscopy and RT-PCR at different timepoints postinjection. Low-dose aphantoxin exposure was associated with chromatin condensation, cell-membrane blebbing, and the appearance of apoptotic bodies. High-dose exposure was associated with cytoplasmic vacuolization, mitochondrial swelling, and expansion of the endoplasmic reticulum. At early timepoints (3 h) many cells exhibited characteristic features of both apoptosis and necrosis. At later timepoints apoptosis appeared to predominate in the low-dose group, whereas necrosis predominated in the high-dose group. RT-PCR revealed that mRNA levels of the apoptosis-related genes encoding p53, Bax, caspase-3, and c-Jun were upregulated after aphantoxin exposure, but there was no evidence of DNA laddering; apoptosis could take place by pathways independent of DNA fragmentation. These results demonstrate that aphantoxin exposure can cause cell death in zebrafish brain tissue, with low doses inducing apoptosis and higher doses inducing necrosis. (C) 2011 Wiley Periodicals, Inc. Environ Toxicol 28: 239-254, 2013. |
| 英文摘要 | Aphanizomenon flos-aquae (A. flos-aquae), a cyanobacterium frequently encountered in water blooms worldwide, is source of neurotoxins known as PSPs or aphantoxins that present a major threat to the environment and to human health. Although the molecular mechanism of PSP action is well known, many unresolved questions remain concerning its mechanisms of toxicity. Aphantoxins purified from a natural isolate of A. flos-aquae DC-1 were analyzed by high-performance liquid chromatography (HPLC), the major component toxins were the gonyautoxins1 and 5 (GTX1 and GTX5, 34.04% and 21.28%, respectively) and the neosaxitoxin (neoSTX, 12.77%). The LD50 of the aphantoxin preparation was determined to be 11.33 mu g/kg (7.75 mu g saxitoxin equivalents (STXeq) per kg) following intraperitoneal injection of zebrafish (Danio rerio). To address the neurotoxicology of the aphantoxin preparation, zebrafish were injected with low and high sublethal doses of A. flos-aquae DC-1 toxins 7.73 and 9.28 mu g /kg (5.3 and 6.4 mu g STXeq/kg, respectively) and brain tissues were analyzed by electron microscopy and RT-PCR at different timepoints postinjection. Low-dose aphantoxin exposure was associated with chromatin condensation, cell-membrane blebbing, and the appearance of apoptotic bodies. High-dose exposure was associated with cytoplasmic vacuolization, mitochondrial swelling, and expansion of the endoplasmic reticulum. At early timepoints (3 h) many cells exhibited characteristic features of both apoptosis and necrosis. At later timepoints apoptosis appeared to predominate in the low-dose group, whereas necrosis predominated in the high-dose group. RT-PCR revealed that mRNA levels of the apoptosis-related genes encoding p53, Bax, caspase-3, and c-Jun were upregulated after aphantoxin exposure, but there was no evidence of DNA laddering; apoptosis could take place by pathways independent of DNA fragmentation. These results demonstrate that aphantoxin exposure can cause cell death in zebrafish brain tissue, with low doses inducing apoptosis and higher doses inducing necrosis. (C) 2011 Wiley Periodicals, Inc. Environ Toxicol 28: 239-254, 2013. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Environmental Sciences ; Toxicology ; Water Resources |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| 关键词[WOS] | CASPASE-ACTIVATED DNASE ; ALKALINE-PHOSPHATASE ACTIVITIES ; ISCHEMIA-REPERFUSION INJURY ; IMMEDIATE-EARLY GENES ; FISH OREOCHROMIS-SP. ; AMYLOID-BETA-PEPTIDE ; C-JUN ; CELL-DEATH ; SODIUM-CHANNELS ; PATHOLOGICAL-CHANGES |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Programs of China [2008CB418002., 2008CB418001]; National Principal Expert Program on treatment and control of water pollution [2009ZX07106-001, 2008ZX07103-004, 2008ZX07103-007] |
| 语种 | 英语 |
| WOS记录号 | WOS:000318576200001 |
| 公开日期 | 2013-10-31 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19323]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Wuhan Univ Technol, Dept Biol, Wuhan 430070, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Delu,Hu, Chunxiang,Wang, Gaohong,et al. Zebrafish Neurotoxicity from Aphantoxins-Cyanobacterial Paralytic Shellfish Poisons (PSPs) from Aphanizomenon flos-aquae DC-1[J]. ENVIRONMENTAL TOXICOLOGY,2013,28(5):239-254. |
| APA | Zhang, Delu,Hu, Chunxiang,Wang, Gaohong,Li, Dunhai,Li, Genbao,&Liu, Yongding.(2013).Zebrafish Neurotoxicity from Aphantoxins-Cyanobacterial Paralytic Shellfish Poisons (PSPs) from Aphanizomenon flos-aquae DC-1.ENVIRONMENTAL TOXICOLOGY,28(5),239-254. |
| MLA | Zhang, Delu,et al."Zebrafish Neurotoxicity from Aphantoxins-Cyanobacterial Paralytic Shellfish Poisons (PSPs) from Aphanizomenon flos-aquae DC-1".ENVIRONMENTAL TOXICOLOGY 28.5(2013):239-254. |
入库方式： OAI收割
来源：水生生物研究所
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