An improved method for determining phytoplankton chlorophyll a concentration without filtration
文献类型：期刊论文
| 作者 | Qin, Hongjie1,2; Li, Shuangshuang1,2; Li, Dunhai1 |
| 刊名 | HYDROBIOLOGIA
 |
| 出版日期 | 2013-05-01 |
| 卷号 | 707期号:1页码:81-95 |
| 关键词 | Chlorophyll a Clay Cyanobacteria Extraction solvent Flocculation Spectrophotometry |
| ISSN号 | 0018-8158 |
| 通讯作者 | Li, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | The common and routine procedure for the quantification of chlorophyll a (chl a) in aquatic studies has a series of steps. Here, we sought to find optimal conditions for phytoplankton cell harvesting, chl a extraction, and chl a measurement and calculation, to find an effective, cost-saving, safe, and environment-friendly procedure for determining phytoplankton chl a concentration. We replaced the traditional GF/C filters with inorganic polymer flocculants (IPFs) and clay for phytoplankton harvesting, and then various solvents (acetone, ethanol, DMF, and DMSO), IPFs (PAC, PFS, and PAFS) and clay were tested for their suitability for chl a extraction, with or without homogenization at different temperatures for different extraction durations. About 0.3-1.0 g l(-1) of PAC or PFSA combined with 1.0-2.5 g l(-1) clay were found to provide optimal conditions in terms of yield and cost for phytoplankton cell harvesting from water samples. Based on our results, we recommend flocculation and centrifugation instead of glass-fiber membrane filtration for harvesting phytoplankton cells from environmental water samples, 95% ethanol for chl a extraction without homogenization and heating, and spectrophotometry to determine chl a concentration. |
| 英文摘要 | The common and routine procedure for the quantification of chlorophyll a (chl a) in aquatic studies has a series of steps. Here, we sought to find optimal conditions for phytoplankton cell harvesting, chl a extraction, and chl a measurement and calculation, to find an effective, cost-saving, safe, and environment-friendly procedure for determining phytoplankton chl a concentration. We replaced the traditional GF/C filters with inorganic polymer flocculants (IPFs) and clay for phytoplankton harvesting, and then various solvents (acetone, ethanol, DMF, and DMSO), IPFs (PAC, PFS, and PAFS) and clay were tested for their suitability for chl a extraction, with or without homogenization at different temperatures for different extraction durations. About 0.3-1.0 g l(-1) of PAC or PFSA combined with 1.0-2.5 g l(-1) clay were found to provide optimal conditions in terms of yield and cost for phytoplankton cell harvesting from water samples. Based on our results, we recommend flocculation and centrifugation instead of glass-fiber membrane filtration for harvesting phytoplankton cells from environmental water samples, 95% ethanol for chl a extraction without homogenization and heating, and spectrophotometry to determine chl a concentration. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology |
| 研究领域[WOS] | Marine & Freshwater Biology |
| 关键词[WOS] | SPECTROPHOTOMETRIC DETERMINATION ; ETHANOL SOLVENTS ; EXTRACTION ; EQUATIONS ; MICROALGAE ; PIGMENTS ; METHANOL ; ACETONE ; BLOOMS ; WASTE |
| 收录类别 | SCI |
| 资助信息 | National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002] |
| 语种 | 英语 |
| WOS记录号 | WOS:000316332300007 |
| 公开日期 | 2013-10-31 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19325]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Hongjie,Li, Shuangshuang,Li, Dunhai. An improved method for determining phytoplankton chlorophyll a concentration without filtration[J]. HYDROBIOLOGIA,2013,707(1):81-95. |
| APA | Qin, Hongjie,Li, Shuangshuang,&Li, Dunhai.(2013).An improved method for determining phytoplankton chlorophyll a concentration without filtration.HYDROBIOLOGIA,707(1),81-95. |
| MLA | Qin, Hongjie,et al."An improved method for determining phytoplankton chlorophyll a concentration without filtration".HYDROBIOLOGIA 707.1(2013):81-95. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。