中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
An improved method for determining phytoplankton chlorophyll a concentration without filtration

文献类型:期刊论文

作者Qin, Hongjie1,2; Li, Shuangshuang1,2; Li, Dunhai1
刊名HYDROBIOLOGIA
出版日期2013-05-01
卷号707期号:1页码:81-95
关键词Chlorophyll a Clay Cyanobacteria Extraction solvent Flocculation Spectrophotometry
ISSN号0018-8158
通讯作者Li, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要The common and routine procedure for the quantification of chlorophyll a (chl a) in aquatic studies has a series of steps. Here, we sought to find optimal conditions for phytoplankton cell harvesting, chl a extraction, and chl a measurement and calculation, to find an effective, cost-saving, safe, and environment-friendly procedure for determining phytoplankton chl a concentration. We replaced the traditional GF/C filters with inorganic polymer flocculants (IPFs) and clay for phytoplankton harvesting, and then various solvents (acetone, ethanol, DMF, and DMSO), IPFs (PAC, PFS, and PAFS) and clay were tested for their suitability for chl a extraction, with or without homogenization at different temperatures for different extraction durations. About 0.3-1.0 g l(-1) of PAC or PFSA combined with 1.0-2.5 g l(-1) clay were found to provide optimal conditions in terms of yield and cost for phytoplankton cell harvesting from water samples. Based on our results, we recommend flocculation and centrifugation instead of glass-fiber membrane filtration for harvesting phytoplankton cells from environmental water samples, 95% ethanol for chl a extraction without homogenization and heating, and spectrophotometry to determine chl a concentration.
英文摘要The common and routine procedure for the quantification of chlorophyll a (chl a) in aquatic studies has a series of steps. Here, we sought to find optimal conditions for phytoplankton cell harvesting, chl a extraction, and chl a measurement and calculation, to find an effective, cost-saving, safe, and environment-friendly procedure for determining phytoplankton chl a concentration. We replaced the traditional GF/C filters with inorganic polymer flocculants (IPFs) and clay for phytoplankton harvesting, and then various solvents (acetone, ethanol, DMF, and DMSO), IPFs (PAC, PFS, and PAFS) and clay were tested for their suitability for chl a extraction, with or without homogenization at different temperatures for different extraction durations. About 0.3-1.0 g l(-1) of PAC or PFSA combined with 1.0-2.5 g l(-1) clay were found to provide optimal conditions in terms of yield and cost for phytoplankton cell harvesting from water samples. Based on our results, we recommend flocculation and centrifugation instead of glass-fiber membrane filtration for harvesting phytoplankton cells from environmental water samples, 95% ethanol for chl a extraction without homogenization and heating, and spectrophotometry to determine chl a concentration.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Marine & Freshwater Biology
研究领域[WOS]Marine & Freshwater Biology
关键词[WOS]SPECTROPHOTOMETRIC DETERMINATION ; ETHANOL SOLVENTS ; EXTRACTION ; EQUATIONS ; MICROALGAE ; PIGMENTS ; METHANOL ; ACETONE ; BLOOMS ; WASTE
收录类别SCI
资助信息National Basic Research and Development Plan of China: "Occurrence mechanism of cyanobacterial blooms in large shallow lakes'' [2008CB418002]
语种英语
WOS记录号WOS:000316332300007
公开日期2013-10-31
源URL[http://ir.ihb.ac.cn/handle/342005/19325]  
专题水生生物研究所_水环境工程研究中心_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Qin, Hongjie,Li, Shuangshuang,Li, Dunhai. An improved method for determining phytoplankton chlorophyll a concentration without filtration[J]. HYDROBIOLOGIA,2013,707(1):81-95.
APA Qin, Hongjie,Li, Shuangshuang,&Li, Dunhai.(2013).An improved method for determining phytoplankton chlorophyll a concentration without filtration.HYDROBIOLOGIA,707(1),81-95.
MLA Qin, Hongjie,et al."An improved method for determining phytoplankton chlorophyll a concentration without filtration".HYDROBIOLOGIA 707.1(2013):81-95.

入库方式: OAI收割

来源:水生生物研究所

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