Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets
文献类型：期刊论文
| 作者 | Kim, Youngchang1,2; Ye, Zi3; Joachimiak, Grazyna1,2; Videau, Patrick4; Young, Jasmine4; Hurd, Kathryn4; Callahan, Sean M.4; Gornicki, Piotr5; Zhao, Jindong3; Haselkorn, Robert5 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2013-05-07 |
| 卷号 | 110期号:19页码:E1716-E1723 |
| 关键词 | heterocyst differentiation mutagenesis X-ray crystallography |
| ISSN号 | 0027-8424 |
| 通讯作者 | Haselkorn, R (reprint author), Univ Chicago, Dept Mol Genet & Cell Biol, 920 E 58Th St, Chicago, IL 60637 USA. |
| 中文摘要 | HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-angstrom resolution, respectively. The highest-resolution structure shows fine details of specific protein-DNA interactions. The lower-resolution structures with longer DNA duplexes have similar interaction patterns and show how the flap domains interact with DNA in a sequence nonspecific fashion. Fifteen of 15 protein-DNA contacts predicted on the basis of the structure were confirmed by single amino acid mutations that abolished binding in vitro and complementation in vivo. A striking feature of the structure is the association of glutamate 71 from each subunit of the HetR dimer with three successive cytosines in each arm of the palindromic target, a feature that is conserved among all known heterocyst-forming cyanobacteria sequenced to date. |
| 英文摘要 | HetR is an essential regulator of heterocyst development in cyanobacteria. Many mutations in HetR render Anabaena incapable of nitrogen fixation. The protein binds to a DNA palindrome upstream of hetP and other genes. We have determined the crystal structures of HetR complexed with palindromic DNA targets, 21, 23, and 29 bp at 2.50-, 3.00-, and 3.25-angstrom resolution, respectively. The highest-resolution structure shows fine details of specific protein-DNA interactions. The lower-resolution structures with longer DNA duplexes have similar interaction patterns and show how the flap domains interact with DNA in a sequence nonspecific fashion. Fifteen of 15 protein-DNA contacts predicted on the basis of the structure were confirmed by single amino acid mutations that abolished binding in vitro and complementation in vivo. A striking feature of the structure is the association of glutamate 71 from each subunit of the HetR dimer with three successive cytosines in each arm of the palindromic target, a feature that is conserved among all known heterocyst-forming cyanobacteria sequenced to date. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | STRAIN PCC 7120 ; HETEROCYST PATTERN-FORMATION ; REPRESSOR-OPERATOR COMPLEX ; CRYSTAL-STRUCTURE ; AMINO-ACIDS ; SP PCC-7120 ; RECOGNITION ; PROTEIN ; DIFFERENTIATION ; BINDING |
| 收录类别 | SCI |
| 资助信息 | National Institutes of Health [GM094585]; National Science Foundation [MCB-1121346]; Ellison Medical Foundation; US Department of Energy, Office of Biological and Environmental Research [DE-AC02-06CH11357]; US Department of Energy Office of Science laboratory [DE-AC02-06CH11357] |
| 语种 | 英语 |
| WOS记录号 | WOS:000319327700004 |
| 公开日期 | 2013-10-31 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19337]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Argonne Natl Lab, Midwest Ctr Struct Genom, Argonne, IL 60439 USA 2.Argonne Natl Lab, Struct Biol Ctr, Argonne, IL 60439 USA 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 4.Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA 5.Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA 6.Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA |
| 推荐引用方式 GB/T 7714 | Kim, Youngchang,Ye, Zi,Joachimiak, Grazyna,et al. Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2013,110(19):E1716-E1723. |
| APA | Kim, Youngchang.,Ye, Zi.,Joachimiak, Grazyna.,Videau, Patrick.,Young, Jasmine.,...&Joachimiak, Andrzej.(2013).Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,110(19),E1716-E1723. |
| MLA | Kim, Youngchang,et al."Structures of complexes comprised of Fischerella transcription factor HetR with Anabaena DNA targets".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110.19(2013):E1716-E1723. |
入库方式： OAI收割
来源：水生生物研究所
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