中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 中科院水生所知识产出（2009年前）
  4. 水生生物分子与细胞生物学研究中心
  5. 期刊论文
Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila

文献类型：期刊论文

作者Cervantes, Marcella D.1; Hamilton, Eileen P.1; Xiong, Jie2; Lawson, Michael J.3; Yuan, Dongxia2; Hadjithomas, Michalis4; Miao, Wei2; Orias, Eduardo1
刊名PLOS BIOLOGY
出版日期2013-03-01
卷号11期号:3页码:e1001518
关键词RNA-SEQ DNA REARRANGEMENTS GENOME PYRIFORMIS SEQUENCE DIFFERENTIATION TRANSFORMATION ELIMINATION INHERITANCE FREQUENCIES
ISSN号1545-7885
通讯作者Cervantes, MD (reprint author), Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA.
中文摘要The unicellular eukaryote Tetrahymena thermophila has seven mating types. Cells can mate only when they recognize cells of a different mating type as non-self. As a ciliate, Tetrahymena separates its germline and soma into two nuclei. During growth the somatic nucleus is responsible for all gene transcription while the germline nucleus remains silent. During mating, a new somatic nucleus is differentiated from a germline nucleus and mating type is decided by a stochastic process. We report here that the somatic mating type locus contains a pair of genes arranged head-to-head. Each gene encodes a mating type-specific segment and a transmembrane domain that is shared by all mating types. Somatic gene knockouts showed both genes are required for efficient non-self recognition and successful mating, as assessed by pair formation and progeny production. The germline mating type locus consists of a tandem array of incomplete gene pairs representing each potential mating type. During mating, a complete new gene pair is assembled at the somatic mating type locus; the incomplete genes of one gene pair are completed by joining to gene segments at each end of germline array. All other germline gene pairs are deleted in the process. These programmed DNA rearrangements make this a fascinating system of mating type determination.
英文摘要The unicellular eukaryote Tetrahymena thermophila has seven mating types. Cells can mate only when they recognize cells of a different mating type as non-self. As a ciliate, Tetrahymena separates its germline and soma into two nuclei. During growth the somatic nucleus is responsible for all gene transcription while the germline nucleus remains silent. During mating, a new somatic nucleus is differentiated from a germline nucleus and mating type is decided by a stochastic process. We report here that the somatic mating type locus contains a pair of genes arranged head-to-head. Each gene encodes a mating type-specific segment and a transmembrane domain that is shared by all mating types. Somatic gene knockouts showed both genes are required for efficient non-self recognition and successful mating, as assessed by pair formation and progeny production. The germline mating type locus consists of a tandem array of incomplete gene pairs representing each potential mating type. During mating, a complete new gene pair is assembled at the somatic mating type locus; the incomplete genes of one gene pair are completed by joining to gene segments at each end of germline array. All other germline gene pairs are deleted in the process. These programmed DNA rearrangements make this a fascinating system of mating type determination.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics
关键词[WOS]RNA-SEQ ; DNA REARRANGEMENTS ; GENOME ; PYRIFORMIS ; SEQUENCE ; DIFFERENTIATION ; TRANSFORMATION ; ELIMINATION ; INHERITANCE ; FREQUENCIES
收录类别SCI
资助信息National Science Foundation, USA [MCB-1025069, U.S. NSF IGERT DGE-02-21715]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-G-6-4]; National Natural Science Foundation of China [31071993]; Tri-Counties Blood Bank Postdoctoral Fellowship
语种英语
WOS记录号WOS:000316794600018
公开日期2013-10-31
源URL[http://ir.ihb.ac.cn/handle/342005/19340]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位1.Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China
3.Univ Calif Santa Barbara, Biomol Sci & Engn Program, Santa Barbara, CA 93106 USA
4.J Craig Venter Inst, Rockville, MD USA
推荐引用方式
GB/T 7714		 Cervantes, Marcella D.,Hamilton, Eileen P.,Xiong, Jie,et al. Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila[J]. PLOS BIOLOGY,2013,11(3):e1001518.
APA Cervantes, Marcella D..,Hamilton, Eileen P..,Xiong, Jie.,Lawson, Michael J..,Yuan, Dongxia.,...&Orias, Eduardo.(2013).Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila.PLOS BIOLOGY,11(3),e1001518.
MLA Cervantes, Marcella D.,et al."Selecting One of Several Mating Types through Gene Segment Joining and Deletion in Tetrahymena thermophila".PLOS BIOLOGY 11.3(2013):e1001518.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。