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Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella

文献类型：期刊论文

作者Chen CongLin1; Sun XiaoYun2; Liao LanJie1; Luo ShaoXiang1; Li ZhouQuan1; Zhang XiaoHua1; Wang YaPing1; Guo QionLin1; Fang Qin2; Dai HePing1
刊名SCIENCE CHINA-LIFE SCIENCES
出版日期2013
卷号56期号:1页码:59-65
关键词grass carp grass carp reovirus (GCRV) IgM single-chain variable fragment (scFv) antigenicity
ISSN号1674-7305
通讯作者Fang, Q (reprint author), Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China.
中文摘要Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na < ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.
英文摘要Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na < ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]TELEOST FISH ; PROTEIN ; AQUAREOVIRUS ; IDENTIFICATION ; EXPRESSION ; ISOTYPE ; VIRION ; VIRUS ; ASSAY ; VP5
收录类别SCI
资助信息National Basic Research Program of China [2009CB118701, 2009CB118704]; National Natural Science Foundation of China [31072233, 31172434]
语种英语
WOS记录号WOS:000313447000008
公开日期2013-10-31
源URL[http://ir.ihb.ac.cn/handle/342005/19345]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China
推荐引用方式
GB/T 7714		 Chen CongLin,Sun XiaoYun,Liao LanJie,et al. Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(1):59-65.
APA Chen CongLin.,Sun XiaoYun.,Liao LanJie.,Luo ShaoXiang.,Li ZhouQuan.,...&Dai HePing.(2013).Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella.SCIENCE CHINA-LIFE SCIENCES,56(1),59-65.
MLA Chen CongLin,et al."Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella".SCIENCE CHINA-LIFE SCIENCES 56.1(2013):59-65.

入库方式： OAI收割

来源：水生生物研究所

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