中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 广州能源研究所
  3. 中国科学院广州能源研究所
Production of Phenylacetylcarbinol via Biotransformation Using the Co-Culture of Candida tropicalis TISTR 5306 and Saccharomyces cerevisiae TISTR 5606 as the Biocatalyst

文献类型：期刊论文

作者Kumar, Anbarasu1,2,3,4; Techapun, Charin1,2,3; Sommanee, Sumeth1,2,3; Mahakuntha, Chatchadaporn1,2,3; Feng, Juan1,2,3; Htike, Su Lwin1,2,3; Khemacheewakul, Julaluk1,2,3; Porninta, Kritsadaporn1,2,3; Phimolsiripol, Yuthana1,2,3; Wang, Wen5
刊名JOURNAL OF FUNGI
出版日期2023-09-01
卷号9期号:9页码:26
关键词phenylacetylcarbinol pyruvate decarboxylase bioethanol Candida tropicalis Saccharomyces cerevisiae biotransformation
DOI10.3390/jof9090928
通讯作者Nunta, Rojarej(quan_rn@hotmail.com) ; Leksawasdi, Noppol(noppol@hotmail.com)
英文摘要Phenylacetylcarbinol (PAC) is a precursor for the synthesis of several pharmaceuticals, including ephedrine, pseudoephedrine, and norephedrine. PAC is commonly produced through biotransformation using microbial pyruvate decarboxylase (PDC) in the form of frozen-thawed whole cells. However, the lack of microorganisms capable of high PDC activity is the main factor in the production of PAC. In addition, researchers are also looking for ways to utilize agro-industrial residues as an inexpensive carbon source through an integrated biorefinery approach in which sugars can be utilized for bioethanol production and frozen-thawed whole cells for PAC synthesis. In the present study, Candida tropicalis, Saccharomyces cerevisiae, and the co-culture of both strains were compared for their biomass and ethanol concentrations, as well as for their volumetric and specific PDC activities when cultivated in a sugarcane bagasse (SCB) hydrolysate medium (SCBHM). The co-culture that resulted in a higher level of PAC (8.65 +/- 0.08 mM) with 26.4 +/- 0.9 g L-1 ethanol production was chosen for further experiments. Biomass production was scaled up to 100 L and the kinetic parameters were studied. The biomass harvested from the bioreactor was utilized as frozen-thawed whole cells for the selection of an initial pyruvate (Pyr)-to-benzaldehyde (Bz) concentration ([Pyr]/[Bz]) ratio suitable for the PAC biotransformation in a single-phase emulsion system. The initial [Pyr]/[Bz] at 100/120 mM resulted in higher PAC levels with 10.5 +/- 0.2 mM when compared to 200/240 mM (8.60 +/- 0.01 mM). A subsequent two-phase emulsion system with Pyr in the aqueous phase, Bz in the organic phase, and frozen-thawed whole cells of the co-culture as the biocatalyst produced a 1.46-fold higher PAC level when compared to a single-phase emulsion system. In addition, the cost analysis strategy indicated preliminary costs of USD 0.82 and 1.01/kg PAC for the single-phase and two-phase emulsion systems, respectively. The results of the present study suggested that the co-culture of C. tropicalis and S. cerevisiae can effectively produce bioethanol and PAC from SCB and would decrease the overall production cost on an industrial scale utilizing the two-phase emulsion system with the proposed multiple-pass strategy.
WOS关键词PYRUVATE DECARBOXYLASE ; ETHANOL-PRODUCTION ; (R)-PHENYLACETYLCARBINOL PRODUCTION ; YEAST ; FERMENTATION ; HYDROLYSATE ; BIOSYNTHESIS ; CHEMISTRY ; STRAINS
资助项目The authors are grateful for the funding support for this research project from the Center of Excellence in Agro Bio-Circular-Green Industry (Agro-BCG) (CoE66-P001), Office of Research Administration (ORA), Faculty of Agro-Industry, Bioprocess Research Clu[CoE66-P001] ; Office of Research Administration (ORA) ; Bioprocess Research Cluster (BRC), CMU ; TISTR
WOS研究方向Microbiology ; Mycology
语种英语
WOS记录号WOS:001073598400001
出版者MDPI
资助机构The authors are grateful for the funding support for this research project from the Center of Excellence in Agro Bio-Circular-Green Industry (Agro-BCG) (CoE66-P001), Office of Research Administration (ORA), Faculty of Agro-Industry, Bioprocess Research Clu ; Office of Research Administration (ORA) ; Bioprocess Research Cluster (BRC), CMU ; TISTR
源URL[http://ir.giec.ac.cn/handle/344007/39853]  
专题中国科学院广州能源研究所
通讯作者Nunta, Rojarej; Leksawasdi, Noppol
作者单位1.Chiang Mai Univ, Fac Agroind, Ctr Excellence Agro Biocircular Green Ind Agro BCG, Sch Agroind, Chiang Mai 50100, Thailand
2.Chiang Mai Univ, Fac Agroind, Sch Agroind, Bioproc Res Cluster BRC, Chiang Mai 50100, Thailand
3.Chiang Mai Univ, Fac Agroind, Chiang Mai 50100, Thailand
4.Periyar Maniammai Inst Sci & Technol Deemed Univ, Dept Biotechnol, Thanjavur 613403, India
5.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
6.Lampang Rajabhat Univ, Fac Agr Technol, Div Food Innovat & Business, Lampang 52100, Thailand
推荐引用方式
GB/T 7714		 Kumar, Anbarasu,Techapun, Charin,Sommanee, Sumeth,et al. Production of Phenylacetylcarbinol via Biotransformation Using the Co-Culture of Candida tropicalis TISTR 5306 and Saccharomyces cerevisiae TISTR 5606 as the Biocatalyst[J]. JOURNAL OF FUNGI,2023,9(9):26.
APA Kumar, Anbarasu.,Techapun, Charin.,Sommanee, Sumeth.,Mahakuntha, Chatchadaporn.,Feng, Juan.,...&Leksawasdi, Noppol.(2023).Production of Phenylacetylcarbinol via Biotransformation Using the Co-Culture of Candida tropicalis TISTR 5306 and Saccharomyces cerevisiae TISTR 5606 as the Biocatalyst.JOURNAL OF FUNGI,9(9),26.
MLA Kumar, Anbarasu,et al."Production of Phenylacetylcarbinol via Biotransformation Using the Co-Culture of Candida tropicalis TISTR 5306 and Saccharomyces cerevisiae TISTR 5606 as the Biocatalyst".JOURNAL OF FUNGI 9.9(2023):26.

入库方式： OAI收割

来源：广州能源研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。