Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels
文献类型：期刊论文
| 作者 | Li, Guangyu1; Yan, Wei2; Qiao, Qin1; Chen, Jun3; Cai, Fei4; He, Yan1; Zhang, Xuezhen1 |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2012-12-21 |
| 卷号 | 77页码:383-393 |
| 关键词 | Microcystin-LR Histopathology Proteomic analysis Immunotoxicity Spleen |
| ISSN号 | 1874-3919 |
| 通讯作者 | Zhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China. |
| 中文摘要 | Microcystin-LR (MCLR), released by toxic cyanobacterial blooms, has received worldwide concerns in the past decades for its hepatotoxicity. Recent studies show that microcystins (MCs) can be accumulated in immune organs and exert notable immunotoxicity. In order to better understand cellular responses in immune tissues disrupted by MCLR treatment, this work mainly focuses on the spleen impairments of rats. After a subchronic 50 d exposure (1 or 10 mu g/kg body weight per day), spleen index, MCLR accumulation, histological change and plasma lysozyme activity were detected in MCLR-treated rat. Results indicated that prolonged exposure of MCLR led to toxin accumulation and caused severe damage in spleen of rats, and eventually impaired the immune functions. To further our understanding of the toxic effects of MCLR on the spleen and the mechanisms behind it, a proteomic analysis was performed to determine the global effects of MCLR on splenetic protein levels. In total, 48 proteins were identified and showed a significant increase or decrease in abundance compared to the control after MCLR exposure. These proteins are mainly involved in immune response, oxidative stress, energetic metabolism and the cytoskeleton assembly, indicating that MCLR exerts complex toxic effects in rat spleen and jointly results in immunotoxicity. (C) 2012 Elsevier B.V. All rights reserved. |
| 英文摘要 | Microcystin-LR (MCLR), released by toxic cyanobacterial blooms, has received worldwide concerns in the past decades for its hepatotoxicity. Recent studies show that microcystins (MCs) can be accumulated in immune organs and exert notable immunotoxicity. In order to better understand cellular responses in immune tissues disrupted by MCLR treatment, this work mainly focuses on the spleen impairments of rats. After a subchronic 50 d exposure (1 or 10 mu g/kg body weight per day), spleen index, MCLR accumulation, histological change and plasma lysozyme activity were detected in MCLR-treated rat. Results indicated that prolonged exposure of MCLR led to toxin accumulation and caused severe damage in spleen of rats, and eventually impaired the immune functions. To further our understanding of the toxic effects of MCLR on the spleen and the mechanisms behind it, a proteomic analysis was performed to determine the global effects of MCLR on splenetic protein levels. In total, 48 proteins were identified and showed a significant increase or decrease in abundance compared to the control after MCLR exposure. These proteins are mainly involved in immune response, oxidative stress, energetic metabolism and the cytoskeleton assembly, indicating that MCLR exerts complex toxic effects in rat spleen and jointly results in immunotoxicity. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEINS ; CARP CTENOPHARYNGODON-IDELLA ; HEAT-SHOCK PROTEINS ; PROTEOMIC ANALYSIS ; HEPATOTOXIC MICROCYSTINS ; INTERMEDIATE-FILAMENTS ; IN-SITU ; EXPRESSION ; FISH ; CELLS |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China (NSFC) [30800160]; Natural Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Natural Science Foundation of Hubei Province of China [2011CDB151]; NSFC [31070457, 81000576] |
| 语种 | 英语 |
| WOS记录号 | WOS:000313535900030 |
| 公开日期 | 2013-10-31 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19296]  |
| 专题 | 水生生物研究所_淡水生态学研究中心_期刊论文 |
| 作者单位 | 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 2.Hubei Acad Agr Sci, Inst Agr Qual Stand & Technol, Wuhan 430064, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 4.Xianning Univ, Coll Med, Dept Pharmacol, Xianning 437100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guangyu,Yan, Wei,Qiao, Qin,et al. Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels[J]. JOURNAL OF PROTEOMICS,2012,77:383-393. |
| APA | Li, Guangyu.,Yan, Wei.,Qiao, Qin.,Chen, Jun.,Cai, Fei.,...&Zhang, Xuezhen.(2012).Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels.JOURNAL OF PROTEOMICS,77,383-393. |
| MLA | Li, Guangyu,et al."Global effects of subchronic treatment of microcystin-LR on rat splenetic protein levels".JOURNAL OF PROTEOMICS 77(2012):383-393. |
入库方式： OAI收割
来源：水生生物研究所
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