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Recycling enzymatic hydrolysis lignin residues saved cellulase in enzymatic hydrolysis of lignocellulose: An insight from cellulase adsorption mechanism

文献类型：期刊论文

作者Zheng, Wenqiu2,3,4; Qin, Yuyue3; Zhuang, Xinshu1,5,6; Xiao, Huaxi2; Liu, Chun2; Fu, Xiangjin2; Lin, Qinlu2; Lan, Tianqing1,2,3,5,6
刊名INDUSTRIAL CROPS AND PRODUCTS
出版日期2024-02-01
卷号208页码:9
关键词Enzymatic hydrolysis lignin residues Cellulase adsorption Molecular docking Interaction force Enzymatic activity Cellulase recycling
ISSN号0926-6690
DOI10.1016/j.indcrop.2023.117884
通讯作者Lan, Tianqing(lantianqing_8019@163.com)
英文摘要Enzymatic hydrolysis lignin residues (EHLRs) of lignocellulose usually adsorbs cellulase, which can be recycled and used to replace parts of cellulase in the hydrolysis process. To understand this phenomenon during enzymatic hydrolysis (EH) of sugarcane bagasse (SCB) treated with sulfite (SPORL) and dilute acid (DA), the adsorption characteristics between lignin and cellulase in EHLRs were investigated, focusing on interaction force at molecular level and enzymatic activity. The results revealed that SPORL-EHLR adsorbed more beta-glucosidases (beta-GLs) through the stronger electrostatic attraction and hydrogen bond force, causing higher cellulase adsorption amount compared to DA-EHLR. Further exploration demonstrated that the cellobiose's catalytic and binding sites on beta-GLs were separated from the binding site of SPORL-EHLR on beta-GLs, resulting in minimal in-hibition of beta-GLs activity when bound to SPORL-EHLR. Furthermore, adding SPORL-EHLR in SCB hydrolysis saved 40% cellulase. This study deepens the understanding of the adsorption behavior between lignin and cellulase.
WOS关键词BIOFUEL PRODUCTION ; BINDING ; PRETREATMENT ; ACID ; CELLOBIOHYDROLASE ; FRACTIONATION ; EXTRACTION ; ENHANCE ; ENZYMES ; WOOD
资助项目International Science and Technol-ogy Innovation Cooperation Program of the National Key Research and Development Plan of China[2021YFE0114400] ; National Natural Science Foundation of China[22168020] ; Hunan Pro-vincial Natural Science Foundation of China[2023JJ30985]
WOS研究方向Agriculture
语种英语
WOS记录号WOS:001132537500001
出版者ELSEVIER
资助机构International Science and Technol-ogy Innovation Cooperation Program of the National Key Research and Development Plan of China ; National Natural Science Foundation of China ; Hunan Pro-vincial Natural Science Foundation of China
源URL[http://ir.giec.ac.cn/handle/344007/40487]  
专题中国科学院广州能源研究所
通讯作者Lan, Tianqing
作者单位1.CAS Key Lab Renewable Energy, 2 Energy Rd, Guangzhou 510640, Peoples R China
2.Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Res Ctr Rice & Byproduct Deep Proc, 498 South Shaoshan Rd, Changsha 410004, Peoples R China
3.Kunming Univ Sci & Technol, Fac Food Sci & Engn, 727,South Jingming Rd, Kunming 650500, Peoples R China
4.Beijing Forestry Univ, Beijing Key Lab Lignocellulos Chem, 35,Tsinghua East Rd, Beijing 100083, Peoples R China
5.Guangdong Prov Key Lab New & Renewable Energy Res, 2 Energy Rd, Guangzhou 510640, Peoples R China
6.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Energy Rd, Guangzhou 510640, Peoples R China
推荐引用方式
GB/T 7714		 Zheng, Wenqiu,Qin, Yuyue,Zhuang, Xinshu,et al. Recycling enzymatic hydrolysis lignin residues saved cellulase in enzymatic hydrolysis of lignocellulose: An insight from cellulase adsorption mechanism[J]. INDUSTRIAL CROPS AND PRODUCTS,2024,208:9.
APA Zheng, Wenqiu.,Qin, Yuyue.,Zhuang, Xinshu.,Xiao, Huaxi.,Liu, Chun.,...&Lan, Tianqing.(2024).Recycling enzymatic hydrolysis lignin residues saved cellulase in enzymatic hydrolysis of lignocellulose: An insight from cellulase adsorption mechanism.INDUSTRIAL CROPS AND PRODUCTS,208,9.
MLA Zheng, Wenqiu,et al."Recycling enzymatic hydrolysis lignin residues saved cellulase in enzymatic hydrolysis of lignocellulose: An insight from cellulase adsorption mechanism".INDUSTRIAL CROPS AND PRODUCTS 208(2024):9.

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来源：广州能源研究所

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