Challenges and Approaches of Culturing the Unculturable Archaea
文献类型：期刊论文
| 作者 | Rafiq, Muhammad2,3; Hassan, Noor4; Rehman, Maliha2; Hayat, Muhammad5; Nadeem, Gullasht2; Hassan, Farwa4; Iqbal, Naveed6,7; Ali, Hazrat4; Zada, Sahib8; Kang, Yingqian1,11 |
| 刊名 | BIOLOGY-BASEL
 |
| 出版日期 | 2023-12-01 |
| 卷号 | 12期号:12页码:19 |
| 关键词 | archaeal cultivation archaeal diversity culturing media culturing methods |
| DOI | 10.3390/biology12121499 |
| 通讯作者 | Rafiq, Muhammad(mr14311@my.bristol.ac.uk) ; Kang, Yingqian(kangyingqian@gmc.edu.cn) |
| 英文摘要 | Simple Summary Living organisms are divided into three main domains: Domain bacteria and domain archaea have prokaryotic unicellular microbes, while domain eukarya have complex eukaryotic organisms. The two domains, eukarya and bacteria, are more extensively studied for the taxonomy, diversity, ecology, physiology, and clinical and biotechnological aspects compared to the archaea. The laboratory cultivation of archaea is more challenging than bacteria and eukaryotic microbes, making archaea less explored than bacteria and eukarya. The obstacles in the isolation of unculturable archaeal species arise from various known and unknown factors, demanding precise physicochemical and environmental conditions for their growth in the laboratory. Contrary to the earlier belief that archaea thrive only in extreme environments, recent reports suggest their ubiquitous presence, similar to bacteria but difficult to isolate. This review demonstrates the importance of archaeal culturing, challenges in cultivation, current approaches, and proposes future recommendations for successful isolation and investigations of the archaea.Abstract Since Carl Woese's discovery of archaea as a third domain of life, numerous archaeal species have been discovered, yet archaeal diversity is poorly characterized. Culturing archaea is complicated, but several queries about archaeal cell biology, evolution, physiology, and diversity need to be solved by culturing and culture-dependent techniques. Increasing interest in demand for innovative culturing methods has led to various technological and methodological advances. The current review explains frequent hurdles hindering uncultured archaea isolation and discusses features for more archaeal cultivation. This review also discusses successful strategies and available media for archaeal culturing, which might be helpful for future culturing practices. |
| WOS关键词 | EXTREMELY HALOPHILIC ARCHAEA ; SP NOV. ; METHANOGENIC ARCHAEA ; MICROBIAL DIVERSITY ; MARINE-SEDIMENTS ; GEN. NOV. ; CULTIVATION ; BACTERIA ; HALOBACTERIUM ; EVOLUTION |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001130952000001 |
| 出版者 | MDPI |
| 源URL | [http://ir.giec.ac.cn/handle/344007/40528]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Rafiq, Muhammad; Kang, Yingqian |
| 作者单位 | 1.Minist Educ Guizhou, Key Lab Environm Pollut Monitoring & Dis Control, Guiyang 550025, Peoples R China 2.Balochistan Univ IT Engn & Management Sci, Fac Life Sci & Informat, Dept Microbiol, Quetta 87100, Pakistan 3.FF Inst Huzhou Co Ltd, Huzhou 313000, Peoples R China 4.Natl Inst Biotechnol, Pakistan Inst Engn & Appl Sci, Genet Engn Coll, Ind Biotechnol Div, Islamabad 44000, Pakistan 5.Shandong Univ, Inst Microbial Technol, State Key Lab Microbial Technol, Jinan 266101, Peoples R China 6.Balochistan Univ IT Engn & Management Sci, Fac Life Sci & Informat, Dept Biotechnol, Quetta 87100, Pakistan 7.Aga Khan Univ, Dept Paediat & Child Hlth, Karachi 74800, Pakistan 8.Chinese Acad Sci, Guangzhou Inst Energy Conservat, Guangzhou 510640, Peoples R China 9.Natl Univ Med Sci, Dept Biol Sci, Rawalpindi 46000, Pakistan 10.Abdul Wali Khan Univ, Dept Microbiol, Garden Campus, Mardan 23200, Pakistan |
| 推荐引用方式 GB/T 7714 | Rafiq, Muhammad,Hassan, Noor,Rehman, Maliha,et al. Challenges and Approaches of Culturing the Unculturable Archaea[J]. BIOLOGY-BASEL,2023,12(12):19. |
| APA | Rafiq, Muhammad.,Hassan, Noor.,Rehman, Maliha.,Hayat, Muhammad.,Nadeem, Gullasht.,...&Jamal, Muhsin.(2023).Challenges and Approaches of Culturing the Unculturable Archaea.BIOLOGY-BASEL,12(12),19. |
| MLA | Rafiq, Muhammad,et al."Challenges and Approaches of Culturing the Unculturable Archaea".BIOLOGY-BASEL 12.12(2023):19. |
入库方式： OAI收割
来源：广州能源研究所
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