Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix)
文献类型：期刊论文
| 作者 | Guo, Wenjie1,2; Yu, Xiaomu1; Tong, Jingou1 |
| 刊名 | CONSERVATION GENETICS RESOURCES
 |
| 出版日期 | 2013-06-01 |
| 卷号 | 5期号:2页码:525-528 |
| 关键词 | Silver carp Microsatellite Polymorphism Bighead carp Cross-species amplification |
| ISSN号 | 1877-7252 |
| 通讯作者 | Tong, JG (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China. |
| 中文摘要 | In this study, we developed 134 novel polymorphic polynucleotide-repeat microsatellite markers for silver carp (Hypophthalmichthys molitrix) and characterized 40 loci for polymorphism and genetic diversity in a test population. The number of alleles per locus ranged from 3 to 19 (mean 9.4), and the average observed and expected heterozygosities were 0.74-0.76, respectively. Thirty-five loci were highly informative (PIC > 0.5) in silver carp population. After Bonferroni adjustments, no pairs of loci showed evidence of significant linkage disequilibrium, and none of the loci significantly deviated from the Hardy-Weinberg equilibrium. All 134 silver carp microsatellite loci could be successfully amplified in bighead carp (Hypophthalmichthys nobilis) with 65 loci being polymorphic, indicating a high transferability of these microsatellites across species. This set of novel highly polymorphic polynucleotide-repeat microsatellites would be powerful tools for population and conservation genetics studies in silver carp and also in its closely-related bighead carp. |
| 英文摘要 | In this study, we developed 134 novel polymorphic polynucleotide-repeat microsatellite markers for silver carp (Hypophthalmichthys molitrix) and characterized 40 loci for polymorphism and genetic diversity in a test population. The number of alleles per locus ranged from 3 to 19 (mean 9.4), and the average observed and expected heterozygosities were 0.74-0.76, respectively. Thirty-five loci were highly informative (PIC > 0.5) in silver carp population. After Bonferroni adjustments, no pairs of loci showed evidence of significant linkage disequilibrium, and none of the loci significantly deviated from the Hardy-Weinberg equilibrium. All 134 silver carp microsatellite loci could be successfully amplified in bighead carp (Hypophthalmichthys nobilis) with 65 loci being polymorphic, indicating a high transferability of these microsatellites across species. This set of novel highly polymorphic polynucleotide-repeat microsatellites would be powerful tools for population and conservation genetics studies in silver carp and also in its closely-related bighead carp. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biodiversity Conservation ; Genetics & Heredity |
| 研究领域[WOS] | Biodiversity & Conservation ; Genetics & Heredity |
| 关键词[WOS] | GENETIC-LINKAGE MAP ; CONSTRUCTION ; POPULATIONS ; MIDDLE ; RIVER |
| 收录类别 | SCI |
| 资助信息 | MOA [200903045]; NSFC [31272647]; MOST 973 of China [2010CB126305] |
| 语种 | 英语 |
| WOS记录号 | WOS:000318293100055 |
| 公开日期 | 2013-10-31 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19306]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Wenjie,Yu, Xiaomu,Tong, Jingou. Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix)[J]. CONSERVATION GENETICS RESOURCES,2013,5(2):525-528. |
| APA | Guo, Wenjie,Yu, Xiaomu,&Tong, Jingou.(2013).Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix).CONSERVATION GENETICS RESOURCES,5(2),525-528. |
| MLA | Guo, Wenjie,et al."Development of 134 novel polynucleotide-repeat microsatellite markers in silver carp (Hypophthalmichthys molitrix)".CONSERVATION GENETICS RESOURCES 5.2(2013):525-528. |
入库方式： OAI收割
来源：水生生物研究所
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