Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)
文献类型：期刊论文
| 作者 | Shi, Yan1; Zhao, Zhe2; Zhu, Xinping1; Chen, Kunci1; Zhang, Qiya3 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 34期号:1页码:273-279 |
| 关键词 | GRIM-19 Epinephelus coioides Tissue distribution Subcellular localization Overexpression |
| ISSN号 | 1050-4648 |
| 通讯作者 | Zhu, XP (reprint author), Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China. |
| 中文摘要 | GRIM-19 is a nuclear encoded subunit of complex I that has been implicated in apoptosis. The protein participates in multiple functions including the innate immune response. GRIM-19 has been studied in humans and other mammals; however, fish GRIM-19 has not been well characterized. In this study, a new GRIM-19 gene, EcGRIM-19, was isolated from the orange-spotted grouper (Epinephelus coioides) cDNA library, which was constructed following LPS treatment. EcGRIM-19 is a 582-bp gene that encodes a 144-amino acid protein. The gene is a true ortholog of mammalian GRIM-19. EcGRIM-19 exhibits ubiquitous and constitutive expression in the different tissues of the orange-spotted grouper. The expression levels of EcGRIM-19 are altered in the gill, spleen, kidney and liver after induction with LPS. The subcellular localization analysis demonstrated that the EcGRIM-19 protein is localized predominantly in the mitochondria. In addition, amino acids 30-50 of the protein are responsible for the mitochondrial localization of EcGRIM-19. The caspase assay demonstrated that the overexpression of GRIM-19 enhanced the cellular sensitivity to interferon(IFN)-beta- and retinoic acid (RA)-induced death in HeLa cells. The data presented in this study are important for further understanding the EcGRIM-19 gene function in fish. (c) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | GRIM-19 is a nuclear encoded subunit of complex I that has been implicated in apoptosis. The protein participates in multiple functions including the innate immune response. GRIM-19 has been studied in humans and other mammals; however, fish GRIM-19 has not been well characterized. In this study, a new GRIM-19 gene, EcGRIM-19, was isolated from the orange-spotted grouper (Epinephelus coioides) cDNA library, which was constructed following LPS treatment. EcGRIM-19 is a 582-bp gene that encodes a 144-amino acid protein. The gene is a true ortholog of mammalian GRIM-19. EcGRIM-19 exhibits ubiquitous and constitutive expression in the different tissues of the orange-spotted grouper. The expression levels of EcGRIM-19 are altered in the gill, spleen, kidney and liver after induction with LPS. The subcellular localization analysis demonstrated that the EcGRIM-19 protein is localized predominantly in the mitochondria. In addition, amino acids 30-50 of the protein are responsible for the mitochondrial localization of EcGRIM-19. The caspase assay demonstrated that the overexpression of GRIM-19 enhanced the cellular sensitivity to interferon(IFN)-beta- and retinoic acid (RA)-induced death in HeLa cells. The data presented in this study are important for further understanding the EcGRIM-19 gene function in fish. (c) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | INDUCED CELL-DEATH ; COMPLEX-I ; ACID ; IDENTIFICATION ; REGULATOR ; APOPTOSIS ; INTERACTS ; PROTEIN ; GROWTH ; FISH |
| 收录类别 | SCI |
| 资助信息 | Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences [LMB091006]; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences [2010FB09]; National Natural Science Foundation of China [41276163, 30901117]; Fundamental Research Funds from CAFS [2012A0403] |
| 语种 | 英语 |
| WOS记录号 | WOS:000314743600032 |
| 公开日期 | 2013-10-31 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/19309]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China 2.Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yan,Zhao, Zhe,Zhu, Xinping,et al. Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):273-279. |
| APA | Shi, Yan,Zhao, Zhe,Zhu, Xinping,Chen, Kunci,&Zhang, Qiya.(2013).Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,34(1),273-279. |
| MLA | Shi, Yan,et al."Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):273-279. |
入库方式： OAI收割
来源：水生生物研究所
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