Pretreatment and enzymatic hydrolysis optimization of lignocellulosic biomass for ethanol, xylitol, and phenylacetylcarbinol co-production using Candida magnoliae
文献类型：期刊论文
| 作者 | Porninta, Kritsadaporn2,3; Khemacheewakul, Julaluk1,3; Techapun, Charin1,3; Phimolsiripol, Yuthana1,3; Jantanasakulwong, Kittisak1,3; Sommanee, Sumeth1,3; Mahakuntha, Chatchadaporn1,3; Feng, Juan1,3; Htike, Su Lwin1,3; Moukamnerd, Churairat1 |
| 刊名 | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
 |
| 出版日期 | 2024-01-18 |
| 卷号 | 11页码:21 |
| 关键词 | sustainability ethanol xylitol phenylacetylcarbinol optimization response surface methodology |
| ISSN号 | 2296-4185 |
| DOI | 10.3389/fbioe.2023.1332185 |
| 通讯作者 | Nunta, Rojarej(noppol.l@cmu.ac.th) ; Leksawasdi, Noppol(rojarej.rn@gmail.com) |
| 英文摘要 | Cellulosic bioethanol production generally has a higher operating cost due to relatively expensive pretreatment strategies and low efficiency of enzymatic hydrolysis. The production of other high-value chemicals such as xylitol and phenylacetylcarbinol (PAC) is, thus, necessary to offset the cost and promote economic viability. The optimal conditions of diluted sulfuric acid pretreatment under boiling water at 95 degrees C and subsequent enzymatic hydrolysis steps for sugarcane bagasse (SCB), rice straw (RS), and corn cob (CC) were optimized using the response surface methodology via a central composite design to simplify the process on the large-scale production. The optimal pretreatment conditions (diluted sulfuric acid concentration (% w/v), treatment time (min)) for SCB (3.36, 113), RS (3.77, 109), and CC (3.89, 112) and the optimal enzymatic hydrolysis conditions (pretreated solid concentration (% w/v), hydrolysis time (h)) for SCB (12.1, 93), RS (10.9, 61), and CC (12.0, 90) were achieved. CC xylose-rich and CC glucose-rich hydrolysates obtained from the respective optimal condition of pretreatment and enzymatic hydrolysis steps were used for xylitol and ethanol production. The statistically significant highest (p <= 0.05) xylitol and ethanol yields were 65% +/- 1% and 86% +/- 2% using Candida magnoliae TISTR 5664. C. magnoliae could statistically significantly degrade (p <= 0.05) the inhibitors previously formed during the pretreatment step, including up to 97% w/w hydroxymethylfurfural, 76% w/w furfural, and completely degraded acetic acid during the xylitol production. This study was the first report using the mixed whole cells harvested from xylitol and ethanol production as a biocatalyst in PAC biotransformation under a two-phase emulsion system (vegetable oil/1 M phosphate (Pi) buffer). PAC concentration could be improved by 2-fold compared to a single-phase emulsion system using only 1 M Pi buffer. |
| WOS关键词 | PYRUVATE DECARBOXYLASE DEACTIVATION ; (R)-PHENYLACETYLCARBINOL PRODUCTION ; SUGARCANE BAGASSE ; CORN COB ; ZYMOMONAS-MOBILIS ; ACID ; FERMENTATION ; KINETICS ; XYLOSE ; PH |
| 资助项目 | Royal Golden Jubilee (RGJ) Ph.D. Programme10.13039/501100012309[PHD/0111/2560] ; National Research Council of Thailand (NRCT)[FF66/042] ; Fundamental Fund 2023, Chiang Mai University (CMU)[CoE66-P001] ; Multidisciplinary and Interdisciplinary School, CMU ; Bioprocess Research Cluster (BRC) ; Office of Research Administration (ORA) ; TISTR |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:001155172600001 |
| 出版者 | FRONTIERS MEDIA SA |
| 资助机构 | Royal Golden Jubilee (RGJ) Ph.D. Programme10.13039/501100012309 ; National Research Council of Thailand (NRCT) ; Fundamental Fund 2023, Chiang Mai University (CMU) ; Multidisciplinary and Interdisciplinary School, CMU ; Bioprocess Research Cluster (BRC) ; Office of Research Administration (ORA) ; TISTR |
| 源URL | [http://ir.giec.ac.cn/handle/344007/40870]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Nunta, Rojarej; Leksawasdi, Noppol |
| 作者单位 | 1.Chiang Mai Univ, Fac Agroind, Chiang Mai, Thailand 2.Chiang Mai Univ, Multidisciplinary & Interdisciplinary Sch, Program Biotechnol, Chiang Mai, Thailand 3.Chiang Mai Univ, Fac Agroind, Sch Agroind, Cluster Agro Biocircular Green Ind Agro BCG, Chiang Mai, Thailand 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou, Peoples R China 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Energy Inst, Key Lab Biofuels, Qingdao, Peoples R China 6.Periyar Maniammai Inst Sci & Technol, Dept Biotechnol, Thanjavur, India 7.Lampang Rajabhat Univ, Fac Agr Technol, Div Food Innovat & Business, Lampang, Thailand |
| 推荐引用方式 GB/T 7714 | Porninta, Kritsadaporn,Khemacheewakul, Julaluk,Techapun, Charin,et al. Pretreatment and enzymatic hydrolysis optimization of lignocellulosic biomass for ethanol, xylitol, and phenylacetylcarbinol co-production using Candida magnoliae[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2024,11:21. |
| APA | Porninta, Kritsadaporn.,Khemacheewakul, Julaluk.,Techapun, Charin.,Phimolsiripol, Yuthana.,Jantanasakulwong, Kittisak.,...&Leksawasdi, Noppol.(2024).Pretreatment and enzymatic hydrolysis optimization of lignocellulosic biomass for ethanol, xylitol, and phenylacetylcarbinol co-production using Candida magnoliae.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,11,21. |
| MLA | Porninta, Kritsadaporn,et al."Pretreatment and enzymatic hydrolysis optimization of lignocellulosic biomass for ethanol, xylitol, and phenylacetylcarbinol co-production using Candida magnoliae".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 11(2024):21. |
入库方式： OAI收割
来源：广州能源研究所
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